Evidence map›Paper›PMID 26046256›Full record

ArticleThe ISME journal2016

Directed assembly of a bacterial quorum.

Matthew D Servinsky, Jessica L Terrell, Chen-Yu Tsao, Hsuan-Chen Wu, David N Quan, Amin Zargar, Patrick C Allen, Christopher M Byrd, Christian J Sund, William E Bentley

Abstract read
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In one paragraph

Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Matthew D ServinskyUS Army Research Laboratory, Sensors and Electron Devices Directorate, Adelphi, MD, USA.
Jessica L TerrellFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Chen-Yu TsaoFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Hsuan-Chen WuFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
David N QuanFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Amin ZargarFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Patrick C AllenUS Army Research Laboratory, Sensors and Electron Devices Directorate, Adelphi, MD, USA.
Christopher M ByrdUS Army Research Laboratory, Sensors and Electron Devices Directorate, Adelphi, MD, USA.
Christian J SundUS Army Research Laboratory, Sensors and Electron Devices Directorate, Adelphi, MD, USA.
William E BentleyFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Many reports have elucidated the mechanisms and consequences of bacterial quorum sensing (QS), a molecular communication system by which bacterial cells enumerate their cell density and organize collective behavior. In few cases, however, the numbers of bacteria exhibiting this collective behavior have been reported, either as a number concentration or a fraction of the whole. Not all cells in the population, for example, take on the collective phenotype. Thus, the specific attribution of the postulated benefit can remain obscure. This is partly due to our inability to independently assemble a defined quorum, for natural and most artificial systems the quorum itself is a consequence of the biological context (niche and signaling mechanisms). Here, we describe the intentional assembly of quantized quorums. These are made possible by independently engineering the autoinducer signal transduction cascade of Escherichia coli (E. coli) and the sensitivity of detector cells so that upon encountering a particular autoinducer level, a discretized sub-population of cells emerges with the desired phenotype. In our case, the emergent cells all express an equivalent amount of marker protein, DsRed, as an indicator of a specific QS-mediated activity. The process is robust, as detector cells are engineered to target both large and small quorums. The process takes about 6 h, irrespective of quorum level. We demonstrate sensitive detection of autoinducer-2 (AI-2) as an application stemming from quantized quorums. We then demonstrate sub-population partitioning in that AI-2-secreting cells can 'call' groups neighboring cells that 'travel' and establish a QS-mediated phenotype upon reaching the new locale.

Indexed as

Quorum SensingEscherichia coliEscherichia coli ProteinsHomoserineLactonesSignal TransductionEscherichia coli ProteinsHomoserineLactonesN-octanoylhomoserine lactone

Identifiers

PMID26046256
PMCPMC4681864

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.