ArticleInternational journal of clinical and experimental pathology2015
Differentiation of immortalized human precartilaginous stem cells into nucleus pulposus-like cells.
Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
3 citing papers in PubMed, 6 citations in OpenAlex.
- Melatonin alleviates oxidative stress-induced injury to nucleus pulposus-derived mesenchymal stem cells through activating PI3K/Akt pathway.Journal of orthopaedic translation · 2023Article
- 1,25(OH)Oxidative medicine and cellular longevity · 2022Article
- MicroRNA-124-3p inhibits the differentiation of precartilaginous stem cells into nucleus pulposus-like cells via targeting FSTL1.Experimental and therapeutic medicine · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveWe aimed to explore the differentiation of immortalized human precartilaginous stem cells (IPSCs) into nucleus pulposus (NP)-like cells induced by transforming growth factor-β1 (TGF-β1) and examine its biological characteristics.
methodsThe IPSCs were seeded onto chitosan/glycerophosphate (C/GP) scaffolds and induced into NP-like cells by adding TGF-β1 under hypoxic conditions. The growth and differentiation of IPSCs were observed, and the formation of glycosaminoglycans (GAG) in the extracellular matrix of differentiating cells was detected by Alcian Blue staining. The expressions of type II collagen and aggrecan genes in NP-like cells were examined by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of Sox9 and β-catenin were analyzed by Western blotting.
resultsThe IPSCs were observed to grow well on the C/GP scaffolds. After 7 days, Alcian Blue staining demonstrated the formation of GAG. The RT-PCR results showed that expression of type II collagen and aggrecan were upregulated compared with control group (P<0.05, P<0.05). Likewise, western blotting results showed that the expression of Sox9 and β-catenin was upregulated compared with control group (P<0.05, P<0.05).
conclusionsThe findings demonstrate that IPSCs could be differentiated into NP-like cells following induction by TGF-β1.
Indexed as
Identifiers
26045789PMC4440098W598790608What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.