ArticleEcotoxicology (London, England)2015
Molecular cloning of class III chitinase gene from Avicennia marina and its expression analysis in response to cadmium and lead stress.
Article in Ecotoxicology (London, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Genome-wide identification of chitinase gene family in Hordeum vulgare: insights into stress response mechanisms and evolutionary dynamics.BMC plant biology · 2025Article
- Overexpression of chitinaseVirulence · 2023Article
- Cloning and Expression of Class I Chitinase Genes from Four Mangrove Species under Heavy Metal Stress.Plants (Basel, Switzerland) · 2023Article
- Molecular Cloning and Expression Analysis of the Typical Class III Chitinase Genes from Three Mangrove Species under Heavy Metal Stress.Plants (Basel, Switzerland) · 2023Article
- Characterization and Expression Analysis of Four Cadmium-Tolerance-Associated Genes ofBiology · 2023Article
- New Evidence of Semi-Mangrove PlantInternational journal of environmental research and public health · 2022Article
- Genetic and molecular mechanisms underlying mangrove adaptations to intertidal environments.iScience · 2022Review
- Article
- Proline metabolism and molecular cloning of AmP5CS in the mangrove Avicennia marina under heat stress.Ecotoxicology (London, England) · 2020Article
- Molecular cloning and expression of AmCDPK from mangrove Avicennia marina under elevated temperature.Ecotoxicology (London, England) · 2020Article
- Morphological Responses and Gene Expression of Grain Amaranth (Plants (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mangrove species have high tolerance to heavy metal pollution. Chitinases have been widely reported as defense proteins in response to heavy metal stress in terrestrial plants. In this study, a full-length cDNA sequence encoding an acidic and basic class III chitinase (AmCHI III) was cloned by using RT-PCR and RACE methods in Avicennia marina. AmCHI III mRNA expression in leaf of A. marina were investigated under Cd, Pb stresses on using real-time quantitative PCR. The deduced AmCHI III protein consists of 302 amino acids, including a signal putative peptide region, and a catalytic domain. Homology modeling of the catalytic domain revealed a typical molecular structure of class III plant chitinases. Results further demonstrated that the regulation of AmCHI III mRNA expression in leaves was strongly dependent on Cd, Pb stresses. AmCHI III mRNA expressions were significantly increased in response to Cd, Pb, and peaked at 7 days Cd-exposure, 7 days Pb-exposure, respectively. AmCHI III mRNA expression exhibited more sensitive to Pb stress than Cd stress. This work was the first time cloing chitinase from A. marina, and it brought evidence on chitinase gene involving in heavy metals (Cd(2+) and Pb(2+)) resistance or detoxification in plants. Further studies including the promoter and upstream regulation, gene over-expression and the response of mangrove chitinases to other stresses will shed more light on the role of chitinase in mangrove plants.
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