ArticleDevelopment genes and evolution2015
Germ line transformation and in vivo labeling of nuclei in Diptera: report on Megaselia abdita (Phoridae) and Chironomus riparius (Chironomidae).
Article in Development genes and evolution, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed.
- Genomic resources for the scuttle fly Megaselia abdita: a model organism for comparative developmental studies in flies.Development (Cambridge, England) · 2025Article
- Article
- Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids.Biology open · 2025Article
- A two-level staging system for the embryonic morphogenesis of the Mediterranean fruit fly (medfly) Ceratitis capitata.PloS one · 2024Article
- Autofluorescent Biomolecules in Diptera: From Structure to Metabolism and Behavior.Molecules (Basel, Switzerland) · 2022Review
- In toto light sheet fluorescence microscopy live imaging datasets of Ceratitis capitata embryonic development.Scientific data · 2022Article
- A Cross-Sectional Survey of Biosafety Professionals Regarding Genetically Modified Insects.Applied biosafety : journal of the American Biological Safety Association · 2020Article
- Decoupling from yolk sac is required for extraembryonic tissue spreading in the scuttle flyeLife · 2018Article
- Article
- Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos.Journal of visualized experiments : JoVE · 2017Article
- Folded gastrulation and T48 drive the evolution of coordinated mesoderm internalization in flies.eLife · 2016Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand how and when developmental traits of the fruit fly Drosophila melanogaster originated during the course of insect evolution, similar traits are functionally studied in variably related satellite species. The experimental toolkit available for relevant fly models typically comprises gene expression and loss as well as gain-of-function analyses. Here, we extend the set of available molecular tools to piggyBac-based germ line transformation in two satellite fly models, Megaselia abdita and Chironomus riparius. As proof-of-concept application, we used a Gateway variant of the piggyBac transposon vector pBac{3xP3-eGFPafm} to generate a transgenic line that expresses His2Av-mCherry as fluorescent nuclear reporter ubiquitously in the gastrulating embryo of M. abdita. Our results open two phylogenetically important nodes of the insect order Diptera for advanced developmental evolutionary genetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.