ArticleJournal of experimental botany2015
Functional analysis of the three HMA4 copies of the metal hyperaccumulator Arabidopsis halleri.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 70 citations in OpenAlex.
- Cis-regulatory elements co-opting core circadian clock regulator CCA1 underlie enhanced expression of HMA4 for metal hyperaccumulation in Arabidopsis halleri.Plant communications · 2025Article
- Zinc translocation from Zn-sufficient to Zn-deficient roots as an adaptation to heterogeneous Zn availability.BMC plant biology · 2025Article
- The Arabidopsis receptor-like kinase WAKL4 limits cadmium uptake via phosphorylation and degradation of NRAMP1 transporter.Nature communications · 2024Article
- Mapping QTL for Mineral Accumulation and Shoot Dry Biomass in Barley under Different Levels of Zinc Supply.International journal of molecular sciences · 2023Article
- ZIP Genes Are Involved in the Retransfer of Zinc Ions during the Senescence of Zinc-Deficient Rice Leaves.International journal of molecular sciences · 2023Article
- Long-term acclimation to cadmium exposure reveals extensive phenotypic plasticity in Chlamydomonas.Plant physiology · 2021Article
- Comparative understanding of metal hyperaccumulation in plants: a mini-review.Environmental geochemistry and health · 2021Review
- Metal and Metalloid Toxicity in Plants: An Overview on Molecular Aspects.Plants (Basel, Switzerland) · 2021Review
- The Role of Aquaporin Overexpression in the Modulation of Transcription of Heavy Metal Transporters under Cadmium Treatment in Poplar.Plants (Basel, Switzerland) · 2020Article
- Zinc allocation to and withinPlant-environment interactions (Hoboken, N.J.) · 2020Article
- di-Cysteine Residues of theFrontiers in plant science · 2020Article
- Bidens pilosa L. hyperaccumulating Cd with different species in soil and the role of EDTA on the hyperaccumulation.Environmental science and pollution research international · 2019Article
- Site-specific regulation of transcriptional responses to cadmium stress in the hyperaccumulator, Sedum alfredii: based on stem parenchymal and vascular cells.Plant molecular biology · 2019Article
- Homology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase.Journal of experimental botany · 2019Article
- di-Cysteine motifs in the C-terminus of plant HMA4 proteins confer nanomolar affinity for zinc and are essential for HMA4 function in vivo.Journal of experimental botany · 2018Article
- Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs.BMC genomics · 2018Article
- Photosynthetic Efficiency as Bioindicator of Environmental Pressure inPlant physiology · 2017Article
- Metal binding to the N-terminal cytoplasmic domain of the PIB ATPase HMA4 is required for metal transport in Arabidopsis.Plant molecular biology · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Arabidopsis halleri, the HMA4 gene has an essential function in Zn/Cd hypertolerance and hyperaccumulation by mediating root-to-shoot translocation of metals. Constitutive high expression of AhHMA4 results from a tandem triplication and cis-activation of the promoter of all three copies. The three AhHMA4 copies possess divergent promoter sequences, but highly conserved coding sequences, and display identical expression profiles in the root and shoot vascular system. Here, an AhHMA4::GFP fusion was expressed under the control of each of the three A. halleri HMA4 promoters in a hma2hma4 double mutant of A. thaliana to individually examine the function of each AhHMA4 copy. The protein showed non-polar localization at the plasma membrane of the root pericycle cells of both A. thaliana and A. halleri. The expression of each AhHMA4::GFP copy complemented the severe Zn-deficiency phenotype of the hma2hma4 mutant by restoring root-to-shoot translocation of Zn. However, each copy had a different impact on metal homeostasis in the A. thaliana genetic background: AhHMA4 copies 2 and 3 were more highly expressed and provided higher Zn tolerance in roots and accumulation in shoots than copy 1, and AhHMA4 copy 3 also increased Cd tolerance in roots. These data suggest a certain extent of functional differentiation among the three A. halleri HMA4 copies, stemming from differences in expression levels rather than in expression profile. HMA4 is a key node of the Zn homeostasis network and small changes in expression level can have a major impact on Zn allocation to root or shoot tissues.
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