ArticleAnnals of botany2015
Flow sorting of C-genome chromosomes from wild relatives of wheat Aegilops markgrafii, Ae. triuncialis and Ae. cylindrica, and their molecular organization.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- Flow Cytometric Analysis and Sorting of Plant Chromosomes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.BMC genomics · 2022Article
- Flow karyotyping of wheat-Frontiers in plant science · 2022Article
- Chromosome analysis and sorting.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Review
- A novel leaf rust resistance gene introgressed from Aegilops markgrafii maps on chromosome arm 2AS of wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Development of Wheat-Frontiers in plant science · 2020Article
- Analysis of Structural Genomic Diversity inFrontiers in plant science · 2020Article
- Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting.The plant genome · 2019Article
- DArTseq-based analysis of genomic relationships among species of tribe Triticeae.Scientific reports · 2018Article
- Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes.Annals of botany · 2018Article
- Molecular and Cytogenetic Characterization of Six Wheat-Frontiers in plant science · 2018Article
- Development of intron targeting (IT) markers specific for chromosome arm 4VS of Haynaldia villosa by chromosome sorting and next-generation sequencing.BMC genomics · 2017Article
- Agronomic Traits and Molecular Marker Identification of Wheat-Frontiers in plant science · 2017Article
- Fluorescence chromosome banding and FISH mapping in perennial ryegrass, Lolium perenne L.BMC genomics · 2016Article
- Introgression of chromosome segments from multiple alien species in wheat breeding lines with wheat streak mosaic virus resistance.Heredity · 2016Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAegilops markgrafii (CC) and its natural hybrids Ae. triuncialis (U(t)U(t)C(t)C(t)) and Ae. cylindrica (D(c)D(c)C(c)C(c)) represent a rich reservoir of useful genes for improvement of bread wheat (Triticum aestivum), but the limited information available on their genome structure and the shortage of molecular (cyto-) genetic tools hamper the utilization of the extant genetic diversity. This study provides the complete karyotypes in the three species obtained after fluorescent in situ hybridization (FISH) with repetitive DNA probes, and evaluates the potential of flow cytometric chromosome sorting.
methodsThe flow karyotypes obtained after the analysis of 4',6-diamidino-2-phenylindole (DAPI)-stained chromosomes were characterized and the chromosome content of the peaks on the flow karyotypes was determined by FISH. Twenty-nine conserved orthologous set (COS) markers covering all seven wheat homoeologous chromosome groups were used for PCR with DNA amplified from flow-sorted chromosomes and genomic DNA. KEY
resultsFISH with repetitive DNA probes revealed that chromosomes 4C, 5C, 7C(t), T6U(t)S.6U(t)L-5C(t)L, 1C(c) and 5D(c) could be sorted with purities ranging from 66 to 91 %, while the remaining chromosomes could be sorted in groups of 2-5. This identified a partial wheat-C-genome homology for group 4 and 5 chromosomes. In addition, 1C chromosomes were homologous with group 1 of wheat; a small segment from group 2 indicated 1C-2C rearrangement. An extensively rearranged structure of chromosome 7C relative to wheat was also detected.
conclusionsThe possibility of purifying Aegilops chromosomes provides an attractive opportunity to investigate the structure and evolution of the Aegilops C genome and to develop molecular tools to facilitate the identification of alien chromatin and support alien introgression breeding in bread wheat.
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