Evidence map›Paper›PMID 26043403›Full record

ArticleJournal of the American Chemical Society2015

A Simple RNA-DNA Scaffold Templates the Assembly of Monofunctional Virus-Like Particles.

Rees F Garmann, Richard Sportsman, Christian Beren, Vinothan N Manoharan, Charles M Knobler, William M Gelbart

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the American Chemical Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
5.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Pleomorphism in Wild-Type and Engineered PP7 Virus-Like Particles.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Article
  3. Single-particle studies of the effects of RNA-protein interactions on the self-assembly of RNA virus particles.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  4. Article
  5. Review
  6. Measurements of the self-assembly kinetics of individual viral capsids around their RNA genome.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  7. Review
  8. Article
  9. A modelling paradigm for RNA virus assembly.Current opinion in virology · 2018
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Rees F Garmann†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Richard Sportsman†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Christian Beren†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Vinothan N Manoharan†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Charles M Knobler†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
William M Gelbart†Department of Chemistry and Biochemistry, ‡California NanoSystems Institute, and §Molecular Biology Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Harvard University · USCalifornia NanoSystems Institute · US

Funding

CHEMISTRY BIOLOGY INTERFACE TRAINING PROGRAMT32GM008496 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MAYNARD, HEATHER D · 1993 to 2019
$6.3M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
NCRR NIH HHS 1S10RR23057NCRR NIH HHS S10 RR023057NIGMS NIH HHS 5T32GM008496NIGMS NIH HHS T32 GM008496
6 · The paper itself

Abstract

Using the components of a particularly well-studied plant virus, cowpea chlorotic mottle virus (CCMV), we demonstrate the synthesis of virus-like particles (VLPs) with one end of the packaged RNA extending out of the capsid and into the surrounding solution. This construct breaks the otherwise perfect symmetry of the capsid and provides a straightforward route for monofunctionalizing VLPs using the principles of DNA nanotechnology. It also allows physical manipulation of the packaged RNA, a previously inaccessible part of the viral architecture. Our synthesis does not involve covalent chemistry of any kind; rather, we trigger capsid assembly on a scaffold of viral RNA that is hybridized at one end to a complementary DNA strand. Interaction of CCMV capsid protein with this RNA-DNA template leads to selective packaging of the RNA portion into a well-formed capsid but leaves the hybridized portion poking out of the capsid through a small hole. We show that the nucleic acid protruding from the capsid is capable of binding free DNA strands and DNA-functionalized colloidal particles. Separately, we show that the RNA-DNA scaffold can be used to nucleate virus formation on a DNA-functionalized surface. We believe this self-assembly strategy can be adapted to viruses other than CCMV.

Indexed as

NanotechnologyBromovirusCapsidDNA, ComplementaryGoldModels, MolecularNanoparticlesRNA, ViralVirionDNA, ComplementaryGoldRNA, Viral

Identifiers

PMID26043403
PMCPMC4694638
OpenAlexW2291547165

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.