ArticleBiosensors & bioelectronics2015
High sensitivity automated multiplexed immunoassays using photonic crystal enhanced fluorescence microfluidic system.
Article in Biosensors & bioelectronics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
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- Activate capture and digital counting (AC + DC) assay for protein biomarker detection integrated with a self-powered microfluidic cartridge.Lab on a chip · 2019Article
- Vertically sheathing laminar flow-based immunoassay using simultaneous diffusion-driven immune reactions.RSC advances · 2019Article
- Glucose biosensor based on open-source wireless microfluidic potentiostat.Sensors and actuators. B, Chemical · 2019Article
- Bioinspired transfer method for the patterning of multiple nanomaterials.RSC advances · 2019Article
- Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization.Sensors (Basel, Switzerland) · 2018Review
- An Automated Microfluidic Assay for Photonic Crystal Enhanced Detection and Analysis of an Antiviral Antibody Cancer Biomarker in Serum.IEEE sensors journal · 2018Article
- Multiplexed efficient on-chip sample preparation and sensitive amplification-free detection of Ebola virus.Biosensors & bioelectronics · 2017Article
- Self-Assembly of Colloidal Photonic Crystals of PS@PNIPAM Nanoparticles and Temperature-Responsive Tunable Fluorescence.Journal of fluorescence · 2016Article
- Optofluidic chips with nanochannels for dynamic molecular detection using enhanced fluorescence.Biomedical optics express · 2016Article
- Opto-Microfluidic Immunosensors: From Colorimetric to Plasmonic.Micromachines · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate a platform that integrates photonic crystal enhanced fluorescence (PCEF) detection of a surface-based microspot fluorescent assay with a microfluidic cartridge to achieve simultaneous goals of high analytic sensitivity (single digit pg/mL), high selectivity, low sample volume, and assay automation. The PC surface, designed to provide optical resonances for the excitation wavelength and emission wavelength of Cyanines 5 (Cy5), was used to amplify the fluorescence signal intensity measured from a multiplexed biomarker microarray. The assay system is comprised of a plastic microfluidic cartridge for holding the PC and an assay automation system that provides a leak-free fluid interface during introduction of a sequence of fluids under computer control. Through the use of the assay automation system and the PC embedded within the microfluidic cartridge, we demonstrate pg/mL-level limits of detection by performing representative biomarker assays for interleukin 3 (IL3) and Tumor Necrosis Factor (TNF-α). The results are consistent with limits of detection achieved without the use of the microfluidic device with the exception that coefficients of variability from spot-to-spot are substantially lower than those obtained by performing assays with manual manipulation of assay liquids. The system's capabilities are compatible with the goal of diagnostic instruments for point-of-care settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.