Evidence map›Paper›PMID 26042622›Full record

ArticleArchives of oral biology2015

Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studies.

Krzysztof Kamocki, Jacques E Nör, Marco C Bottino

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of oral biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
7.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 40 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Krzysztof KamockiDepartment of Restorative Dentistry-Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), 1121W. Michigan St. (DS270B), Indianapolis, IN 46202, USA.
Jacques E NörDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA.
Marco C BottinoDepartment of Restorative Dentistry-Division of Dental Biomaterials, Indiana University School of Dentistry (IUSD), 1121W. Michigan St. (DS270B), Indianapolis, IN 46202, USA. Electronic address: mbottino@iu.edu.
Indiana University – Purdue University Indianapolis · USUniversity of Michigan–Ann Arbor · US

Funding

Mechanisms of dental pulp stem cell differentiation into functional endotheliumR01DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NOR, JACQUES EDUARDO · 2011 to 2021
$3.2M
Regulation of dental pulp stem cell fateR37DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jacques Eduardo Nor · 2024 to 2026
$1.2M
A Novel Multilayered Membrane for Periodontal RegenerationK08DE023552 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BOTTINO, MARCO C · 2013 to 2017
$716k
NIDCR NIH HHS K08 DE023552NIDCR NIH HHS R37 DE021410
6 · The paper itself

Abstract

objectiveA combination of antibiotics, including but not limited to metronidazole (MET) and ciprofloxacin (CIP), has been indicated to eradicate bacteria in necrotic immature permanent teeth prior to regenerative procedures. It has been shown clinically that antibiotic pastes may lead to substantial stem cell death. The aim of this study was to synthesise scaffolds containing various concentrations of CIP to enhance cell viability while preserving antimicrobial properties.

designPolydioxanone (PDS)-based electrospun scaffolds were processed with decreasing CIP concentrations (25-1 wt.%) and morphologically evaluated using scanning electron microscopy (SEM). Cytotoxicity assays were performed to determine whether the amount of CIP released from the scaffolds would lead to human dental pulp stem cell (hDPSC) toxicity. Similarly, WST-1 assays were performed to evaluate the impact of CIP release on hDPSC proliferation. Pure PDS scaffolds and saturated double antibiotic solution MET/CIP (DAP) served as both positive and negative controls, respectively. Antibacterial efficacy against E. faecalis (Ef) was tested.

resultsA significant decrease in hDPSC' viability at concentrations 5-25 wt.% was observed. However, concentrations below 5wt.% did not impair cell viability. Data from the WST-1 assays indicated no detrimental impact on cell proliferation for scaffolds containing 2.5 wt.% CIP or less. Significant antimicrobial properties were seen for CIP-scaffolds at lower concentrations (i.e., 1 and 2.5 wt.%).

conclusionThe obtained data demonstrated that a reduced concentration of CIP incorporated into PDS-based scaffolds maintains its antimicrobial properties while enhancing viability and proliferation of dental pulp stem cells.

Indexed as

Anti-Infective AgentsCell ProliferationCell SurvivalCiprofloxacinDental PulpHumansIn Vitro TechniquesMetronidazoleMicrobial Sensitivity TestsMicroscopy, Electron, ScanningStem CellsTissue ScaffoldsAnti-Infective AgentsCiprofloxacinMetronidazoleAntibioticsCytocompatibilityEndodonticsRegenerationScaffoldsStem cells

Identifiers

PMID26042622
PMCPMC5338688
OpenAlexW1857208282

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.