ArticleFrontiers in microbiology2015
A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 136 papers.
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Who cites it
136 citing papers in PubMed.
- Distinct Environmental DNA States Reveal Biodiversity and Transport Patterns Across Alpine Watersheds.Molecular ecology resources · 2026Article
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- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.Applied and environmental microbiology · 2026Article
- Co-prevalence of SARS-CoV-2 and Noroviruses in Wastewater Collection and Treatment Facilities of the Eastern Cape Region, South Africa: An Exacerbated Public and Environmental Health Risk Concern.Food and environmental virology · 2026Article
- Identification of bacterial candidates that promote the growth of the seagrassbioRxiv : the preprint server for biology · 2026Article
- Pathogens on High-Touch Surfaces in an Arid Megacity: A Longitudinal Molecular Surveillance Study.Microorganisms · 2026Article
- Assessment of the effectiveness of host depletion techniques for profiling fish skin microbiomes and metagenomic analysis.Microbiology spectrum · 2026Article
- Warming Causes a Decline in Baltic Sea Coastal Sediment Microbial Abundance.Environmental microbiology · 2026Article
- Dynamic changes in protist community composition along a surface water-groundwater transect in the Danube wetland Lobau, Vienna, Austria.Frontiers in microbiology · 2026Article
- State-Specific Extraction of Environmental DNA: Spike-and-Recovery Controls to Validate and Optimise Extraction Protocols.Environmental microbiology · 2025Article
- The impact of chemical reactivity on the quality and stability of RNA-LNP pharmaceuticals.Nature reviews. Chemistry · 2025Review
- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.medRxiv : the preprint server for health sciences · 2025Article
- Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys.Advanced biology · 2025Article
- Impacts of environmentally relevant concentrations of antibiotic cocktails on the skin microbiome of Eurasian carp (Cyprinus carpio).Animal microbiome · 2025Article
- Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing.BMC research notes · 2025Article
- Deciphering chromate tolerance and reduction ability of an indigenous Bacillus strain isolated from polluted pond sludge for chromium bioremediation.Scientific reports · 2025Article
- Complementary Rhizosphere Microbial Strategies Drive Functional Specialization in Coastal Halophyte Succession: Differential Adaptation ofMicroorganisms · 2025Article
- Resilience of deep aquifer microbial communities to seasonal hydrological fluctuations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
76 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permutations of all nucleic acid extraction steps were compared. The final modular protocol is suitable for extractions from igneous rock, air, water, and sediments. Sediments range from high-biomass, organic rich coastal samples to samples from the most oligotrophic region of the world's oceans and the deepest borehole ever studied by scientific ocean drilling. Extraction yields of DNA and RNA are higher than with widely used commercial kits, indicating an advantage to optimizing extraction procedures to match specific sample characteristics. The ability to separate soluble extracellular DNA pools without cell lysis from intracellular and particle-complexed DNA pools may enable new insights into the cycling and preservation of DNA in environmental samples in the future. A general protocol is outlined, along with recommendations for optimizing this general protocol for specific sample types and research goals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.