Evidence map›Paper›PMID 26041979›Full record

ArticleCancer cell international2015

MicroR-545 enhanced radiosensitivity via suppressing Ku70 expression in Lewis lung carcinoma xenograft model.

Chen Liao, Wei Xiao, Nuo Zhu, Zhiyuan Liu, Jiu Yang, Yanhu Wang, Mei Hong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Chen LiaoDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Wei XiaoDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Nuo ZhuDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Zhiyuan LiuDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Jiu YangDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Yanhu WangDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Mei HongDepartment of Radiotherapy, Nanjing Chest Hospital Affiliated to Southeast University, Guangzhou Road, No215, P.R, Nanjing, Jiangsu 210029 China.
Southeast University · CNNanjing Chest Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveRadiotherapy is an important therapeutic method for lung cancer. However, in clinical situations, cellular resistance to radiotherapy is a significant component of tumor treatment failure. Thus, clarification in cellular mechanism underlying radiosensitivity of cancer cell is urgently needed. In this study, we established a radiation model of Lewis lung carcinoma in C57BL/6 mice and investigated the possible signaling molecule involved in this process.

methodsC57BL/6 mice were subcutaneously transplanted with Lewis lung carcinoma cells and locally irradiated followed by measurement in tumor volume. Levels of miR-545 and Ku70 mRNA expression were determined by using Quantitative Real-Time PCR. Expression of Ku70 was determined by using western blot assay. Cell viability was analyzed by MTT assay. Cell apoptosis was examined by using TUNEL assay.

resultsIn mice bearing Lewis lung carcinoma tumor, local radiotherapy suppressed tumor growth as well as enhanced expression of miR-545 and downregulated Ku70 level. Inhibition of miR-545 expression reduced radiosensitivity of Lewis tumor. In vitro Lewis lung carcinoma cells experiment, we observed that miR-545 regulated Ku70 expression by targeting Ku70 3'UTR and this process was involved in radiotherapy. This was demonstrated by result of cell proliferation assay in which irradiation reduced apoptosis of cells was mediated by miR-545 inactivation which was reversed by Ku70 silence.

conclusionmiR-545 increased radiosensitivity of Lewis lung carcinoma via inhibiting Ku70 expression.

Indexed as

C57BL/6 miceKu70Lewis lung carcinoma cellsmiR-545Radiotherapy

Identifiers

PMID26041979
PMCPMC4453103
OpenAlexW2171573545

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.