ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2015
Circadian Activators Are Expressed Days before They Initiate Clock Function in Late Pacemaker Neurons from Drosophila.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- Article
- Tissue-specific CLOCK isoforms modulate circadian feedback loops to govern reproductive fitness in Drosophila.Cellular and molecular life sciences : CMLS · 2025Article
- Synaptic targets of circadian clock neurons influence core clock parameters.Science advances · 2025Article
- Alternative splicing ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- The Drosophila circadian clock gene cycle controls the development of clock neurons.PLoS genetics · 2024Article
- Spatiotemporal changes in Netrin/Dscam1 signaling dictate axonal projection direction ineLife · 2024Article
- The CoREST complex regulates multiple histone modifications temporal-specifically in clock neurons.Open biology · 2024Article
- Alternative splicing ofbioRxiv : the preprint server for biology · 2024Article
- Article
- Developmental emergence of sleep rhythms enables long-term memory inScience advances · 2023Article
- Dorsal clock neurons iniScience · 2021Article
- Light Stimuli and Circadian Clock Affect Neural Development inFrontiers in cell and developmental biology · 2021Article
- Proteomic analysis of Drosophila CLOCK complexes identifies rhythmic interactions with SAGA and Tip60 complex component NIPPED-A.Scientific reports · 2020Article
- Involvement of Melatonin in the Regulation of the Circadian System in Crayfish.International journal of molecular sciences · 2018Review
- Neuroanatomical details of the lateral neurons of Drosophila melanogaster support their functional role in the circadian system.The Journal of comparative neurology · 2018Article
- A sleep state ineLife · 2018Article
- CLOCK stabilizes CYCLE to initiate clock function inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Drosophila Clock Is Required in Brain Pacemaker Neurons to Prevent Premature Locomotor Aging Independently of Its Circadian Function.PLoS genetics · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Circadian pacemaker neurons in the Drosophila brain control daily rhythms in locomotor activity. These pacemaker neurons can be subdivided into early or late groups depending on whether rhythms in period (per) and timeless (tim) expression are initiated at the first instar (L1) larval stage or during metamorphosis, respectively. Because CLOCK-CYCLE (CLK-CYC) heterodimers initiate circadian oscillator function by activating per and tim transcription, a Clk-GFP transgene was used to mark when late pacemaker neurons begin to develop. We were surprised to see that CLK-GFP was already expressed in four of five clusters of late pacemaker neurons during the third instar (L3) larval stage. CLK-GFP is only detected in postmitotic neurons from L3 larvae, suggesting that these four late pacemaker neuron clusters are formed before the L3 larval stage. A GFP-cyc transgene was used to show that CYC, like CLK, is also expressed exclusively in pacemaker neurons from L3 larval brains, demonstrating that CLK-CYC is not sufficient to activate per and tim in late pacemaker neurons at the L3 larval stage. These results suggest that most late pacemaker neurons develop days before novel factors activate circadian oscillator function during metamorphosis.
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