ArticleProceedings of the National Academy of Sciences of the United States of America2015
Integrated 3D view of postmating responses by the Drosophila melanogaster female reproductive tract, obtained by micro-computed tomography scanning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 70 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
70 citing papers in PubMed, 156 citations in OpenAlex.
- A novel female reproductive organ differentiates insemination and postmating responses in bedbugs.Molecular biology and evolution · 2026Article
- Female membrane proteins regulate postmating ovulation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evolutionary morphology of genital spines informed by puncture mechanics.Proceedings. Biological sciences · 2025Article
- Correlated evolution of elaborate intromission mechanics during copulation between the sexes in leaf beetles.Journal of the Royal Society, Interface · 2025Article
- Behaviour and reproduction of Drosophila melanogaster exposed to 3.6 GHz radio-frequency electromagnetic fields.PloS one · 2025Article
- A sexually transmitted sugar orchestrates reproductive responses to nutritional stress.Nature communications · 2024Article
- How does a fly die? Insights into ageing from the pathophysiology of Drosophila mortality.GeroScience · 2024Review
- Structural analysis of the female reptile reproductive system by micro-computed tomography and optical coherence tomography†.Biology of reproduction · 2024Article
- Roles of herbivorous insects salivary proteins.Heliyon · 2024Review
- Article
- A developmental atlas of male terminalia across twelve species ofFrontiers in cell and developmental biology · 2024Article
- Transcriptional programs are activated and microRNAs are repressed within minutes after mating in the Drosophila melanogaster female reproductive tract.BMC genomics · 2023Article
- Article
- Piezo-mediated mechanosensation contributes to stabilizing copulation posture and reproductive success iniScience · 2023Article
- A symbiotic physical niche in Drosophila melanogaster regulates stable association of a multi-species gut microbiota.Nature communications · 2023Article
- Resolving between novelty and homology in the rapidly evolving phallus of Drosophila.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Article
- Time series transcriptome analysis implicates the circadian clock in theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Standardized terminology and visual atlas of the external morphology and terminalia for the genusFly · 2022Review
- Review
- Assessing Anatomical Changes in Male Reproductive Organs in Response to Larval Crowding Using Micro-computed Tomography Imaging.Neotropical entomology · 2022Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Physiological changes in females during and after mating are triggered by seminal fluid components in conjunction with female-derived molecules. In insects, these changes include increased egg production, storage of sperm, and changes in muscle contraction within the reproductive tract (RT). Such postmating changes have been studied in dissected RT tissues, but understanding their coordination in vivo requires a holistic view of the tissues and their interrelationships. Here, we used high-resolution, multiscale micro-computed tomography (CT) scans to visualize and measure postmating changes in situ in the Drosophila female RT before, during, and after mating. These studies reveal previously unidentified dynamic changes in the conformation of the female RT that occur after mating. Our results also reveal how the reproductive organs temporally shift in concert within the confines of the abdomen. For example, we observed chiral loops in the uterus and in the upper common oviduct that relax and constrict throughout sperm storage and egg movement. We found that specific seminal fluid proteins or female secretions mediate some of the postmating changes in morphology. The morphological movements, in turn, can cause further changes due to the connections among organs. In addition, we observed apparent copulatory damage to the female intima, suggesting a mechanism for entry of seminal proteins, or other exogenous components, into the female's circulatory system. The 3D reconstructions provided by high-resolution micro-CT scans reveal how male and female molecules and anatomy interface to carry out and coordinate mating-dependent changes in the female's reproductive physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.