ArticleInternational journal of legal medicine2016
Postmortem degradation of skeletal muscle proteins: a novel approach to determine the time since death.
Article in International journal of legal medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 3 of them syntheses that pooled it.
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Who cites it
46 citing papers in PubMed, 3 syntheses or guidelines pooled it, 90 citations in OpenAlex.
- Proteomic profiling of bone for the estimation of post-mortem interval and post-mortem submersion interval: a systematic review.International journal of legal medicine · 2026Pooled it
- Forensic applications of postmortem protein analysis in estimating the time since death: a systematic review.International journal of legal medicine · 2026Pooled it
- "Omics" and Postmortem Interval Estimation: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Estimating time of death from smartwatch data: initial empirical results.Scientific reports · 2026Article
- Post-mortem supravital skeletal muscle excitability for early post-mortem interval estimation: a scoping review and methodological map.Forensic science, medicine, and pathology · 2026Review
- Article
- Peptide ratios for post-mortem interval estimation using targeted liquid chromatography triple quadrupole mass spectrometry.International journal of legal medicine · 2026Article
- Protein degradation in human skeletal muscle: advancing postmortem interval estimation under natural outdoor conditions.International journal of legal medicine · 2026Article
- Death in Epilepsy: Ictogenic Vs. Cardiogenic Mechanisms and Immunoassays in Postmortem Interval Estimation - Case Report.Maedica · 2026Article
- Factors influencing the progression of post-mortem changes between scene and autopsy.Scientific reports · 2026Article
- Recent Advances in Forensic Medicine at the Horizon of Omics Sciences and New Biomarkers for Postmortem Interval Estimation.Maedica · 2025Review
- Descriptive analysis of protein expression variations during pupal development ofForensic sciences research · 2025Article
- Exploring human hair degradation: A preliminary study for estimating time-since-death.International journal of legal medicine · 2025Review
- RedefiningFrontiers in microbiology · 2025Review
- Postmortem interval estimation of time since death: impact of non-histone binding proteins, immunohistochemical, and histopathological changes in vivo.Journal of medicine and life · 2024Article
- Morphological changes and protein degradation during the decomposition process of pig cadavers placed outdoors or in tents-a pilot study.Forensic science, medicine, and pathology · 2024Article
- The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review.International journal of molecular sciences · 2024Review
- Effect of temporary freezing on postmortem protein degradation patterns.International journal of legal medicine · 2023Article
- Forensic Proteomics for the Discovery of NewInternational journal of molecular sciences · 2023Article
- Estimating the time of human decomposition based on skeletal muscle biopsy samples utilizing an untargeted LC-MS/MS-based proteomics approach.Analytical and bioanalytical chemistry · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estimating the time since death is a very important aspect in forensic sciences which is pursued by a variety of methods. The most precise method to determine the postmortem interval (PMI) is the temperature method which is based on the decrease of the body core temperature from 37 °C. However, this method is only useful in the early postmortem phase (~0-36 h). The aim of the present work is to develop an accurate method for PMI determination beyond this present limit. For this purpose, we used sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, and casein zymography to analyze the time course of degradation of selected proteins and calpain activity in porcine biceps femoris muscle until 240 h postmortem (hpm). Our results demonstrate that titin, nebulin, desmin, cardiac troponin T, and SERCA1 degraded in a regular and predictable fashion in all samples investigated. Similarly, both the native calpain 1 and calpain 2 bands disintegrate into two bands subsequently. This degradation behavior identifies muscular proteins and enzymes as promising substrates for future molecular-based PMI determination technologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.