ArticleProceedings. Biological sciences2015
Ectopic expression of ecdysone oxidase impairs tissue degeneration in Bombyx mori.
Article in Proceedings. Biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- Draper-ATG3 Interaction Positively Regulates Autophagy to Mediate Silk Gland Degradation in Bombyx mori.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Wingless strain created using binary transgenic CRISPR/Cas9 alleviates concerns about mass rearing of Hermetia illucens.Communications biology · 2024Article
- Article
- A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.Nature communications · 2023Article
- Article
- Advances in Editing Silkworms (Insects · 2021Review
- The receptor tyrosine kinase torso regulates ecdysone homeostasis to control developmental timing in Bombyx mori.Insect science · 2021Article
- A palmitoyltransferase Approximated gene Bm-app regulates wing development in Bombyx mori.Insect science · 2020Article
- Molecular Disruption of Ion Transport Peptide Receptor Results in Impaired Water Homeostasis and Developmental Defects inFrontiers in physiology · 2020Article
- CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori.Insect science · 2019Article
- Cell death during complete metamorphosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Regulation of the Larval Transcriptome ofFrontiers in physiology · 2019Article
- Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.PLoS genetics · 2018Article
- The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm,The Journal of biological chemistry · 2017Article
- Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus.Journal of virology · 2017Article
- Resources and Recommendations for Using Transcriptomics to Address Grand Challenges in Comparative Biology.Integrative and comparative biology · 2016Review
- 20-Hydroxyecdysone (20E) Primary Response Gene E75 Isoforms Mediate Steroidogenesis Autoregulation and Regulate Developmental Timing in Bombyx.The Journal of biological chemistry · 2016Article
- Changes in 30K protein synthesis during delayed degeneration of the silk gland by a caspase-dependent pathway in a Bombyx (silkworm) mutant.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metamorphosis in insects includes a series of programmed tissue histolysis and remolding processes that are controlled by two major classes of hormones, juvenile hormones and ecdysteroids. Precise pulses of ecdysteroids (the most active ecdysteroid is 20-hydroxyecdysone, 20E), are regulated by both biosynthesis and metabolism. In this study, we show that ecdysone oxidase (EO), a 20E inactivation enzyme, expresses predominantly in the midgut during the early pupal stage in the lepidopteran model insect, Bombyx mori. Depletion of BmEO using the transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/RNA-guided Cas9 nucleases) system extended the duration of the final instar larval stage. Ubiquitous transgenic overexpression of BmEO using the Gal4/UAS system induced lethality during the larval-pupal transition. When BmEO was specifically overexpressed in the middle silk gland (MSG), degeneration of MSG at the onset of metamorphosis was blocked. Transmission electron microscope and LysoTracker analyses showed that the autophagy pathway in MSG is inhibited by BmEO ectopic expression. Furthermore, RNA-seq analysis revealed that the genes involved in autophagic cell death and the mTOR signal pathway are affected by overexpression of BmEO. Taken together, BmEO functional studies reported here provide insights into ecdysone regulation of tissue degeneration during metamorphosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.