ArticleNucleic acids research2015
Sequence-independent characterization of viruses based on the pattern of viral small RNAs produced by the host.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 148 citations in OpenAlex.
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- The genomic diversity of arthropod-specific viruses reinforces the continental distribution pattern of Aedes aegypti.Parasites & vectors · 2025Article
- Virome profiling of Culex tarsalis through small RNA-seq: A challenge of suboptimal samples.PLoS neglected tropical diseases · 2025Article
- Virome sequencing and analysis of Aedes aegypti and Aedes albopictus from ecologically different sites in the Philippines.Parasites & vectors · 2025Article
- Integrative multi-omics approach identified emerging viral threat in Theobroma cacao plants.BMC plant biology · 2025Article
- Adaptive evolution of traits for parasitism and pathogen transmission potential in bat flies.National science review · 2025Article
- Insect-specific RNA viruses detection in Field-Caught Aedes aegypti mosquitoes from Argentina using NGS technology.PLoS neglected tropical diseases · 2025Article
- Diversity and transmissibility of RNA viruses in the small brown planthopper,Journal of virology · 2024Article
- Genetic Characterization of Two Novel Insect-Infecting Negative-Sense RNA Viruses Identified in a Leaf Beetle,Insects · 2024Article
- Evolutionary immunology to explore original antiviral strategies.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Virome profiling of fig wasps (Ceratosolen spp.) reveals virus diversity spanning four realms.Virology · 2024Article
- Influence of dengue virus serotypes on the abundance ofJournal of virology · 2024Article
- Conserved untranslated regions of multipartite viruses: Natural markers of novel viral genomic components and tags of viral evolution.Virus evolution · 2024Article
- Interaction of Mesonivirus and Negevirus with arboviruses and the RNAi response in Culex tarsalis-derived cells.Parasites & vectors · 2023Article
- Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response.Scientific reports · 2023Article
- Composition and global distribution of the mosquito virome - A comprehensive database of insect-specific viruses.One health (Amsterdam, Netherlands) · 2023Article
- Review
- The virome of the invasive Asian bush mosquitoVirus evolution · 2023Article
- Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Virus surveillance in vector insects is potentially of great benefit to public health. Large-scale sequencing of small and long RNAs has previously been used to detect viruses, but without any formal comparison of different strategies. Furthermore, the identification of viral sequences largely depends on similarity searches against reference databases. Here, we developed a sequence-independent strategy based on virus-derived small RNAs produced by the host response, such as the RNA interference pathway. In insects, we compared sequences of small and long RNAs, demonstrating that viral sequences are enriched in the small RNA fraction. We also noted that the small RNA size profile is a unique signature for each virus and can be used to identify novel viral sequences without known relatives in reference databases. Using this strategy, we characterized six novel viruses in the viromes of laboratory fruit flies and wild populations of two insect vectors: mosquitoes and sandflies. We also show that the small RNA profile could be used to infer viral tropism for ovaries among other aspects of virus biology. Additionally, our results suggest that virus detection utilizing small RNAs can also be applied to vertebrates, although not as efficiently as to plants and insects.
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