ArticleBMC research notes2015
Transient and stable transformation of Ceratopteris richardii gametophytes.
Article in BMC research notes, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 50 citations in OpenAlex.
- Cell division and lineage dynamics during antheridium differentiation and male gametophyte development in Ceratopteris richardii.Communications biology · 2026Article
- Sex-type specification and meristem development in fern gametophytes.Discover developmental biology · 2026Review
- Cellular basis of ABA-induced de novo meristem formation and sex-type conversion in Ceratopteris gametophytes.The Plant journal : for cell and molecular biology · 2025Article
- Functions and Regulation of HAM Family Genes in Meristems During Gametophyte and Sporophyte Generations.Plant, cell & environment · 2025Review
- The cellular basis of meristem development in fern gametophytes.Biochemical Society transactions · 2025Review
- The rise of CLAVATA: evidence for CLAVATA3 and WOX signaling in the fern gametophyte.The Plant journal : for cell and molecular biology · 2025Article
- Efficient gene editing of a model fern species through gametophyte-based transformation.Plant physiology · 2024Article
- A conserved GRAS-domain transcriptional regulator links meristem indeterminacy to sex determination in Ceratopteris gametophytes.Current biology : CB · 2024Article
- Cell Division and Meristem Dynamics in Fern Gametophytes.Plants (Basel, Switzerland) · 2023Article
- What can hornworts teach us?Frontiers in plant science · 2023Review
- Polarized distribution of extracellular nucleotides promotes gravity-directed polarization of development in spores ofFrontiers in plant science · 2023Article
- Positional cues and cell division dynamics drive meristem development and archegonium formation in Ceratopteris gametophytes.Communications biology · 2022Article
- Cell growth dynamics in two types of apical meristems in fern gametophytes.The Plant journal : for cell and molecular biology · 2022Article
- Vascular function of the T3/modern clade WUSCHEL-Related HOMEOBOX transcription factor genes predate apical meristem-maintenance function.BMC plant biology · 2022Article
- The biology ofeLife · 2022Article
- Quantitative live-imaging reveals the dynamics of apical cells during gametophyte development in ferns.Quantitative plant biology · 2022Article
- Transcriptional analysis ofFrontiers in plant science · 2022Article
- Charting the genomic landscape of seed-free plants.Nature plants · 2021Review
- Article
- A fern WUSCHEL-RELATED HOMEOBOX gene functions in both gametophyte and sporophyte generations.BMC plant biology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFerns, being vascular yet seedless, present unparalleled opportunities to investigate important questions regarding the evolution and development of land plants. Ceratopteris richardii, a diploid, homosporous fern has been advanced as a model fern system; however, the tenuous ability to transform the genome of this fern greatly limited its usefulness as a model organism. Here we report a simple and reliable Agrobacterium-mediated method for generating transient and stable transformants of mature C. richardii gametophytes.
resultsTransformation success was achieved by enzyme treatment that partially digested the cell walls of mature gametophytes to facilitate Agrobacteria infection. Co-incubation of Agrobacteria with enzymatically treated gametophytes was sufficient to generate transient transformants at a frequency of nearly 90% under optimal conditions. Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T0 gametophytes on selective media.
conclusionsThis transformation method will allow for the immediate observation of phenotypes in the haploid gametophytes of transformed plants, as well as the generation of stably transformed C. richardii lines for further analysis. Transformation capability will greatly facilitate gene functional studies in C. richardii, more fully realizing the potential of this model fern species. These protocols may be adapted to other plant species that are recalcitrant to Agrobacterium-mediated transformation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.