ArticleBMC biotechnology2015
Improved detection of Escherichia coli and coliform bacteria by multiplex PCR.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Article
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- Camel plasma as an innovative non-antibiotic therapy forVeterinary world · 2026Article
- Detection of carbapenem resistance among third-generation cephalosporin-resistant Enterobacterales from small-scale poultry farms in peri-urban Lusaka, Zambia.Access microbiology · 2026Article
- Molecular Detection of Extended-Spectrum Beta-Lactamase (ESBL) Genes inInternational journal of food science · 2026Article
- Using wastewater surveillance to investigate community-level differences in antibacterial resistance in a major urban center, USA.Applied and environmental microbiology · 2025Article
- Antimicrobial Resistance Phenotypes and Genotypes ofAntibiotics (Basel, Switzerland) · 2025Article
- Novel degenerate PCR primers targeting the β-galactosidase gene for broad-spectrum identification of lactose-degrading bacteria.World journal of microbiology & biotechnology · 2025Article
- Bacteriological quality of fresh and processed black soldier flyMicrobiology spectrum · 2025Article
- Occurrence of cefotaxime-resistant and beta-lactamase-producing Enterobacterales in poultry from small-scale farms in the Copperbelt province of Zambia.JAC-antimicrobial resistance · 2025Article
- A TaqMan qPCR for precise detection and quantification of diarrheagenic Escherichia coli.Scientific reports · 2025Article
- Development of a PCR-dipstick DNA chromatography-based tool for the detection of CTX-M- and TEM-producing Escherichia coli and Klebsiella pneumoniae isolated from patients in Kafue and Katete districts of Zambia.BMC infectious diseases · 2025Article
- Enhancing RT-PCR Throughput and Sensitivity through Large-Scale Sample Pooling Using a Nano-Hybrid Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- Role of gut microbiome in developing necrotizing enterocolitis.Folia microbiologica · 2025Article
- Multidrug-Resistant VirulentInternational journal of microbiology · 2025Article
- Development of triplex assay for simultaneous detection ofJournal of food science and technology · 2024Article
- Novel multiplex-PCR test forMicrobiology spectrum · 2024Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe presence of coliform bacteria is routinely assessed to establish the microbiological safety of water supplies and raw or processed foods. Coliforms are a group of lactose-fermenting Enterobacteriaceae, which most likely acquired the lacZ gene by horizontal transfer and therefore constitute a polyphyletic group. Among this group of bacteria is Escherichia coli, the pathogen that is most frequently associated with foodborne disease outbreaks and is often identified by β-glucuronidase enzymatic activity or by the redundant detection of uidA by PCR. Because a significant fraction of essential E. coli genes are preserved throughout the bacterial kingdom, alternative oligonucleotide primers for specific E. coli detection are not easily identified.
resultsIn this manuscript, two strategies were used to design oligonucleotide primers with differing levels of specificity for the simultaneous detection of total coliforms and E. coli by multiplex PCR. A consensus sequence of lacZ and the orphan gene yaiO were chosen as targets for amplification, yielding 234 bp and 115 bp PCR products, respectively.
conclusionsThe assay designed in this work demonstrated superior detection ability when tested with lab collection and dairy isolated lactose-fermenting strains. While lacZ amplicons were found in a wide range of coliforms, yaiO amplification was highly specific for E. coli. Additionally, yaiO detection is non-redundant with enzymatic methods.
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