ArticleJournal of cell science2015
Rho-kinase-dependent actin turnover and actomyosin disassembly are necessary for mouse spinal neural tube closure.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis.Current biology : CB · 2026Article
- Planar polarization of endogenous ADIP during Xenopus neurulation.Biology open · 2026Article
- Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Discovering the unexpected: Insights into the dynamics of mouse neural tube closure revealed by time-lapse imaging.Developmental biology · 2026Review
- Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems.Cell reports · 2025Article
- Wnt-dependent mechanism of the apical constriction of roof plate cells in developing mouse spinal cord.Frontiers in cell and developmental biology · 2025Article
- Mechanics of cell sheets: plectin as an integrator of cytoskeletal networks.Open biology · 2025Review
- Retinoic acid induces human gastruloids with posterior embryo-like structures.Nature cell biology · 2024Article
- Induction andbioRxiv : the preprint server for biology · 2024Article
- Deriving early single-rosette brain organoids from human pluripotent stem cells.Stem cell reports · 2023Article
- Article
- Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages.Nature communications · 2023Article
- The surface ectoderm exhibits spatially heterogenous tension that correlates with YAP localisation during spinal neural tube closure in mouse embryos.Cells & development · 2023Article
- Dual mechanism underlying failure of neural tube closure in the Zic2 mutant mouse.Disease models & mechanisms · 2023Article
- Synchronisation of apical constriction and cell cycle progression is a conserved behaviour of pseudostratified neuroepithelia informed by their tissue geometry.Developmental biology · 2023Article
- Pathogenesis of neural tube defects: The regulation and disruption of cellular processes underlying neural tube closure.WIREs mechanisms of disease · 2022Review
- Serum exosomal coronin 1A and dynamin 2 as neural tube defect biomarkers.Journal of molecular medicine (Berlin, Germany) · 2022Article
- Won't You be My Neighbor: How Epithelial Cells Connect Together to Build Global Tissue Polarity.Frontiers in cell and developmental biology · 2022Review
- High glucose causes developmental abnormalities in neuroepithelial cysts with actin and HK1 distribution changes.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The cytoskeleton is widely considered essential for neurulation, yet the mouse spinal neural tube can close despite genetic and non-genetic disruption of the cytoskeleton. To investigate this apparent contradiction, we applied cytoskeletal inhibitors to mouse embryos in culture. Preventing actomyosin cross-linking, F-actin assembly or myosin II contractile activity did not disrupt spinal closure. In contrast, inhibiting Rho kinase (ROCK, for which there are two isoforms ROCK1 and ROCK2) or blocking F-actin disassembly prevented closure, with apical F-actin accumulation and adherens junction disturbance in the neuroepithelium. Cofilin-1-null embryos yielded a similar phenotype, supporting the hypothesis that there is a key role for actin turnover. Co-exposure to Blebbistatin rescued the neurulation defects caused by RhoA inhibition, whereas an inhibitor of myosin light chain kinase, ML-7, had no such effect. We conclude that regulation of RhoA, Rho kinase, LIM kinase and cofilin signalling is necessary for spinal neural tube closure through precise control of neuroepithelial actin turnover and actomyosin disassembly. In contrast, actomyosin assembly and myosin ATPase activity are not limiting for closure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.