ArticleProceedings of the National Academy of Sciences of the United States of America2015
In vitro model for lytic replication, latency, and transformation of an oncogenic alphaherpesvirus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 48 citations in OpenAlex.
- Marek's disease virus-1 unique gene LORF1 is involved in viral replication and MDV-1/Md5-induced atrophy of the bursa of Fabricius.PLoS pathogens · 2025Article
- Article
- The First Record of an Aggressive Form of Ocular Tumour Enhanced by Marek's Disease Virus Infection in Layer Flock in Al-Najaf, Iraq.Veterinary medicine international · 2024Article
- Article
- Temperature-induced reactivation of Marek's disease virus-transformed T cellsFrontiers in veterinary science · 2023Article
- The Importance of the Bursa of Fabricius, B Cells and T Cells for the Pathogenesis of Marek's Disease: A Review.Viruses · 2022Review
- Marek's Disease Virus Virulence Genes Encode Circular RNAs.Journal of virology · 2022Article
- Article
- Visualization of Marek's Disease Virus Genomes in Living Cells during Lytic Replication and Latency.Viruses · 2022Article
- A Cell Culture System to Investigate Marek's Disease Virus Integration into Host Chromosomes.Microorganisms · 2021Article
- Marek's disease virus prolongs survival of primary chicken B-cells by inducing a senescence-like phenotype.PLoS pathogens · 2021Article
- The dominantly expressed class II molecule from a resistant MHC haplotype presents only a few Marek's disease virus peptides by using an unprecedented binding motif.PLoS biology · 2021Article
- The Evaluation of Cellular Immunity to Avian Viral Diseases: Methods, Applications, and Challenges.Frontiers in microbiology · 2021Review
- Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance.PLoS pathogens · 2020Article
- Article
- Revisiting cellular immune response to oncogenic Marek's disease virus: the rising of avian T-cell immunity.Cellular and molecular life sciences : CMLS · 2020Review
- Review
- Glutaminolysis and Glycolysis Are Essential for Optimal Replication of Marek's Disease Virus.Journal of virology · 2020Article
- IFNα and IFNγ Impede Marek's Disease Progression.Viruses · 2019Article
- Marek's Disease Virus Infection of Natural Killer Cells.Microorganisms · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marek's disease virus (MDV) is an alphaherpesvirus that causes deadly T-cell lymphomas in chickens and serves as a natural small animal model for virus-induced tumor formation. In vivo, MDV lytically replicates in B cells that transfer the virus to T cells in which the virus establishes latency. MDV also malignantly transforms CD4+ T cells with a T(reg) signature, ultimately resulting in deadly lymphomas. No in vitro infection system for primary target cells of MDV has been available due to the short-lived nature of these cells in culture. Recently, we characterized cytokines and monoclonal antibodies that promote survival of cultured chicken B and T cells. We used these survival stimuli to establish a culture system that allows efficient infection of B and T cells with MDV. We were able to productively infect with MDV B cells isolated from spleen, bursa or blood cultured in the presence of soluble CD40L. Virus was readily transferred from infected B to T cells stimulated with an anti-TCRαVβ1 antibody, thus recapitulating the in vivo situation in the culture dish. Infected T cells could then be maintained in culture for at least 90 d in the absence of TCR stimulation, which allowed the establishment of MDV-transformed lymphoblastoid cell lines (LCL). The immortalized cells had a signature comparable to MDV-transformed CD4+ α/β T cells present in tumors. In summary, we have developed a novel in vitro system that precisely reflects the life cycle of an oncogenic herpesivrus in vivo and will allow us to investigate the interaction between virus and target cells in an easily accessible system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.