ArticleScientific reports2015
Surface Mn(II) oxidation actuated by a multicopper oxidase in a soil bacterium leads to the formation of manganese oxide minerals.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 67 citations in OpenAlex.
- Comparative transcriptomics and scarless genome editing uncover a periplasmicApplied and environmental microbiology · 2026Article
- Synergy between reactive manganese species and enzymes drives litter decomposition hotspots.Nature communications · 2026Article
- DistinguishingmSystems · 2026Article
- Deciphering the Structure and Genetic Basis of Adaptive Mechanism of Soil Microbial Communities in a Manganese Electrolysis Plant.Microorganisms · 2025Article
- BacteriumBiological characteristics and oxidation performance of a new manganese-oxidizing bacteria: Pseudochrobactrum saccharolyticum.World journal of microbiology & biotechnology · 2025Article
- Article
- Carbon Metabolism of a Soilborne Mn(II)-OxidizingInternational journal of molecular sciences · 2022Article
- From manganese oxidation to water oxidation: assembly and evolution of the water-splitting complex in photosystem II.Photosynthesis research · 2022Review
- Biogenic metal nanoparticles with microbes and their applications in water treatment: a review.Environmental science and pollution research international · 2022Review
- Synthesis of MnO/C/CoScience and technology of advanced materials · 2021Article
- Removal of Mn(II) by a nitrifying bacterium Acinetobacter sp. AL-6: efficiency and mechanisms.Environmental science and pollution research international · 2021Article
- Synthesis of MnO/C/NiO-Doped Porous Multiphasic Composites for Lithium-Ion Batteries by Biomineralized Mn Oxides from EngineeredNanomaterials (Basel, Switzerland) · 2021Article
- Mn oxide formation by phototrophs: Spatial and temporal patterns, with evidence of an enzymatic superoxide-mediated pathway.Scientific reports · 2019Article
- Metal release and sequestration from black slate mediated by a laccase of Schizophyllum commune.Environmental science and pollution research international · 2019Article
- Mechanistic insights into manganese oxidation of a soil-borne Mn(II)-oxidizing Escherichia coli strain by global proteomic and genetic analyses.Scientific reports · 2017Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this manuscript, we report that a bacterial multicopper oxidase (MCO266) catalyzes Mn(II) oxidation on the cell surface, resulting in the surface deposition of Mn(III) and Mn(IV) oxides and the gradual formation of bulky oxide aggregates. These aggregates serve as nucleation centers for the formation of Mn oxide micronodules and Mn-rich sediments. A soil-borne Escherichia coli with high Mn(II)-oxidizing activity formed Mn(III)/Mn(IV) oxide deposit layers and aggregates under laboratory culture conditions. We engineered MCO266 onto the cell surfaces of both an activity-negative recipient and wild-type strains. The results confirmed that MCO266 governs Mn(II) oxidation and initiates the formation of deposits and aggregates. By contrast, a cell-free substrate, heat-killed strains, and intracellularly expressed or purified MCO266 failed to catalyze Mn(II) oxidation. However, purified MCO266 exhibited Mn(II)-oxidizing activity when combined with cell outer membrane component (COMC) fractions in vitro. We demonstrated that Mn(II) oxidation and aggregate formation occurred through an oxygen-dependent biotic transformation process that requires a certain minimum Mn(II) concentration. We propose an approximate electron transfer pathway in which MCO266 transfers only one electron to convert Mn(II) to Mn(III) and then cooperates with other COMC electron transporters to transfer the other electron required to oxidize Mn(III) to Mn(IV).
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