ArticleACS chemical biology2015
Mapping Microbial Response Metabolomes for Induced Natural Product Discovery.
Article in ACS chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Discovery of natural products that modulate signaling in patient-derived cells.RSC chemical biology · 2026Article
- Griseolutein T from Streptomyces seoulensis, newly identified via combined-culture with Tsukamurella pulmonis, as an efficacious therapeutic agent against multidrug-resistant bacteria.The Journal of antibiotics · 2025Article
- Co-cultivation strategies for natural product discovery from actinomycetes: unlocking silent secondary metabolism with mycolic acid-containing bacteria.World journal of microbiology & biotechnology · 2025Review
- Unlocking hidden treasures: the evolution of high-throughput mass spectrometry in screening for cryptic natural products.Natural product reports · 2025Review
- Exploring new natural products by utilizing untapped secondary metabolic pathways in actinomycetes.Journal of natural medicines · 2025Review
- Strategies for Actinobacteria Isolation, Cultivation, and Metabolite Production that Are Biologically Important.ACS omega · 2025Review
- Multiplexed activity metabolomics for isolation of filipin macrolides from a hypogean actinomycete.The Journal of antibiotics · 2025Article
- Bacteria from the Amycolatopsis genus associated with a toxic bird secrete protective secondary metabolites.Nature communications · 2024Article
- The Discovery of Weddellamycin, a Tricyclic Polyene Macrolactam Antibiotic from an Antarctic Deep-Sea-DerivedMarine drugs · 2024Article
- Manipulation and epigenetic control of silent biosynthetic pathways in actinobacteria.World journal of microbiology & biotechnology · 2024Review
- Genome mining for macrolactam-encoding gene clusters allowed for the network-guided isolation of β-amino acid-containing cyclic derivatives and heterologous production of ciromicin A.Communications chemistry · 2023Article
- Small molecule inducers of actinobacteria natural product biosynthesis.Journal of industrial microbiology & biotechnology · 2023Review
- Elicitation for activation of the actinomycete genome's cryptic secondary metabolite gene clusters.RSC advances · 2023Review
- Polyene Macrolactams from Marine and Terrestrial Sources: Structure, Production Strategies, Biosynthesis and Bioactivities.Marine drugs · 2022Review
- Guidelines for metabolomics-guided transposon mutagenesis for microbial natural product discovery.Methods in enzymology · 2022Article
- MetEx, a Metabolomics Explorer Application for Natural Product Discovery.ACS chemical biology · 2021Article
- Metabolomics and genomics in natural products research: complementary tools for targeting new chemical entities.Natural product reports · 2021Review
- A Natural Product Chemist's Guide to Unlocking Silent Biosynthetic Gene Clusters.Annual review of biochemistry · 2021Review
- Discovery of novel secondary metabolites encoded in actinomycete genomes through coculture.Journal of industrial microbiology & biotechnology · 2021Review
- Natural products in drug discovery: advances and opportunities.Nature reviews. Drug discovery · 2021Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Intergeneric microbial interactions may originate a significant fraction of secondary metabolic gene regulation in nature. Herein, we expose a genomically characterized Nocardiopsis strain, with untapped polyketide biosynthetic potential, to intergeneric interactions via coculture with low inoculum exposure to Escherichia, Bacillus, Tsukamurella, and Rhodococcus. The challenge-induced responses of extracted metabolites were characterized via multivariate statistical and self-organizing map (SOM) analyses, revealing the magnitude and selectivity engendered by the limiting case of low inoculum exposure. The collected inventory of cocultures revealed substantial metabolomic expansion in comparison to monocultures with nearly 14% of metabolomic features in cocultures undetectable in monoculture conditions and many features unique to coculture genera. One set of SOM-identified responding features was isolated, structurally characterized by multidimensional NMR, and revealed to comprise previously unreported polyketides containing an unusual pyrrolidinol substructure and moderate and selective cytotoxicity. Designated ciromicin A and B, they are detected across mixed cultures with intergeneric preferences under coculture conditions. The structural novelty of ciromicin A is highlighted by its ability to undergo a diastereoselective photochemical 12-π electron rearrangement to ciromicin B at visible wavelengths. This study shows how organizing trends in metabolomic responses under coculture conditions can be harnessed to characterize multipartite cultures and identify previously silent secondary metabolism.
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