ArticleBiophysical journal2015
Crystal structures of the L1, L2, N, and O states of pharaonis halorhodopsin.
Article in Biophysical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Light-driven anion-pumping rhodopsin with unique cytoplasmic anion-release mechanism.The Journal of biological chemistry · 2024Article
- Direct detection of the chloride release and uptake reactions of Natronomonas pharaonis halorhodopsin.The Journal of biological chemistry · 2024Article
- Mechanisms of inward transmembrane proton translocation.Nature structural & molecular biology · 2023Article
- Structural insights into light-driven anion pumping in cyanobacteria.Nature communications · 2022Article
- Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Crystallization of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Functional Mechanism of ClAdvances in experimental medicine and biology · 2021Review
- Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.Advances in experimental medicine and biology · 2021Review
- Unique ClBiophysics and physicobiology · 2021Article
- Spectroscopic Characterization of Halorhodopsin Reconstituted into Nanodisks Using Native Lipids.Biophysical journal · 2020Article
- Article
- Effect of a bound anion on the structure and dynamics of halorhodopsin fromStructural dynamics (Melville, N.Y.) · 2019Article
- Existence of two O-like intermediates in the photocycle ofBiophysics and physicobiology · 2017Article
- Chimeric microbial rhodopsins for optical activation of Gs-proteins.Biophysics and physicobiology · 2017Article
- A Unique Light-Driven Proton Transportation Signal in Halorhodopsin from Natronomonas pharaonis.Biophysical journal · 2016Article
- Crystal structure of Halobacterium salinarum halorhodopsin with a partially depopulated primary chloride-binding site.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif.Nature communications · 2016Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Halorhodopsin from Natronomonas pharaonis (pHR) functions as a light-driven halide ion pump. In the presence of halide ions, the photochemical reaction of pHR is described by the scheme: K→ L1 → L2 → N → O → pHR' → pHR. Here, we report light-induced structural changes of the pHR-bromide complex observed in the C2 crystal. In the L1-to-L2 transition, the bromide ion that initially exists in the extracellular vicinity of retinal moves across the retinal Schiff base. Upon the formation of the N state with a bromide ion bound to the cytoplasmic vicinity of the retinal Schiff base, the cytoplasmic half of helix F moves outward to create a water channel in the cytoplasmic interhelical space, whereas the extracellular half of helix C moves inward. During the transition from N to an N-like reaction state with retinal assuming the 13-cis/15-syn configuration, the translocated bromide ion is released into the cytoplasmic medium. Subsequently, helix F relaxes into its original conformation, generating the O state. Anion uptake from the extracellular side occurs when helix C relaxes into its original conformation. These structural data provide insight into the structural basis of unidirectional anion transport.
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