ArticleProceedings of the National Academy of Sciences of the United States of America2015
Two-tiered coupling between flowing actin and immobilized N-cadherin/catenin complexes in neuronal growth cones.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Neurons maintain sequential order during radial migration by DSCAM-facilitated antagonism of N-Cadherin.bioRxiv : the preprint server for biology · 2025Article
- Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue.Nature communications · 2024Article
- Adhesion-clutch between DCC and netrin-1 mediates netrin-1-induced axonal haptotaxis.Frontiers in molecular neuroscience · 2024Article
- Two phases for centripetal migration of Drosophila melanogaster follicle cells: initial ingression followed by epithelial migration.Development (Cambridge, England) · 2023Article
- Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.Human molecular genetics · 2022Article
- Multi-Dimensional Spectral Single Molecule Localization Microscopy.Frontiers in bioinformatics · 2022Review
- Advanced imaging and labelling methods to decipher brain cell organization and function.Nature reviews. Neuroscience · 2021Review
- FluoSim: simulator of single molecule dynamics for fluorescence live-cell and super-resolution imaging of membrane proteins.Scientific reports · 2020Article
- Cadherins regulate nuclear topography and function of developing ocular motor circuitry.eLife · 2020Article
- Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.Cell reports · 2020Article
- Vangl2 acts at the interface between actin and N-cadherin to modulate mammalian neuronal outgrowth.eLife · 2020Article
- Forces to Drive Neuronal Migration Steps.Frontiers in cell and developmental biology · 2020Review
- TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering.Cell reports · 2019Article
- Using Single-Protein Tracking to Study Cell Migration.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Article
- Defective Gpsm2/GαNature communications · 2017Article
- Release activity-dependent control of vesicle endocytosis by the synaptic adhesion molecule N-cadherin.Scientific reports · 2017Article
- Gene silencing NMII promotes axonal regeneration against contusive spinal cord injury in rats.International journal of clinical and experimental pathology · 2017Article
- Local Arp2/3-dependent actin assembly modulates applied traction force during apCAM adhesion site maturation.Molecular biology of the cell · 2017Article
- Organization and dynamics of the actin cytoskeleton during dendritic spine morphological remodeling.Cellular and molecular life sciences : CMLS · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuronal growth cones move forward by dynamically connecting actin-based motility to substrate adhesion, but the mechanisms at the individual molecular level remain unclear. We cultured primary neurons on N-cadherin-coated micropatterned substrates, and imaged adhesion and cytoskeletal proteins at the ventral surface of growth cones using single particle tracking combined to photoactivated localization microscopy (sptPALM). We demonstrate transient interactions in the second time scale between flowing actin filaments and immobilized N-cadherin/catenin complexes, translating into a local reduction of the actin retrograde flow. Normal actin flow on micropatterns was rescued by expression of a dominant negative N-cadherin construct competing for the coupling between actin and endogenous N-cadherin. Fluorescence recovery after photobleaching (FRAP) experiments confirmed the differential kinetics of actin and N-cadherin, and further revealed a 20% actin population confined at N-cadherin micropatterns, contributing to local actin accumulation. Computer simulations with relevant kinetic parameters modeled N-cadherin and actin turnover well, validating this mechanism. Such a combination of short- and long-lived interactions between the motile actin network and spatially restricted adhesive complexes represents a two-tiered clutch mechanism likely to sustain dynamic environment sensing and provide the force necessary for growth cone migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.