ArticleEmerging microbes & infections2014
Burkholderia pseudomallei in soil samples from an oceanarium in Hong Kong detected using a sensitive PCR assay.
Article in Emerging microbes & infections, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 20 citations in OpenAlex.
- Probable airborne transmission ofEmerging microbes & infections · 2023Article
- Rational design of an acidic erythritol (ACER) medium for the enhanced isolation of the environmental pathogenFrontiers in microbiology · 2023Article
- Genome-wide transposon mutagenesis analysis ofFrontiers in cellular and infection microbiology · 2022Article
- Environmental Factors Associated With Soil Prevalence of the Melioidosis PathogenFrontiers in microbiology · 2022Article
- No hints for abundance of Bacillus anthracis and Burkholderia pseudomallei in 100 environmental samples from Cameroon.European journal of microbiology & immunology · 2021Article
- The first imported case of melioidosis in a patient in central China.Emerging microbes & infections · 2019Article
- Melioidosis in Hong Kong.Tropical medicine and infectious disease · 2018Review
- Article
- Evaluation of consensus method for the culture ofWellcome open research · 2018Article
- The melioidosis agent Burkholderia pseudomallei and related opportunistic pathogens detected in faecal matter of wildlife and livestock in northern Australia.Epidemiology and infection · 2016Article
- Metabolomic Profiling of Plasma from Melioidosis Patients Using UHPLC-QTOF MS Reveals Novel Biomarkers for Diagnosis.International journal of molecular sciences · 2016Article
- Evaluation of the Bruker Biotyper Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry System for Identification of Clinical and Environmental Isolates of Burkholderia pseudomallei.Frontiers in microbiology · 2016Article
- Laboratory diagnosis of melioidosis: past, present and future.Experimental biology and medicine (Maywood, N.J.) · 2015Review
- Metabolomic profiling of Burkholderia pseudomallei using UHPLC-ESI-Q-TOF-MS reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway.Cell & bioscience · 2015Article
- Melioidosis mycotic aneurysm: An uncommon complication of an uncommon disease.Respiratory medicine case reports · 2015Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Melioidosis, caused by Burkholderia pseudomallei, is an emerging infectious disease with an expanding geographical distribution. Although assessment of the environmental load of B. pseudomallei is important for risk assessment in humans or animals in endemic areas, traditional methods of bacterial culture for isolation have low sensitivities and are labor-intensive. Using a specific polymerase chain reaction (PCR) assay targeting a Tat domain protein in comparison with a bacterial culture method, we examined the prevalence of B. pseudomallei in soil samples from an oceanarium in Hong Kong where captive marine mammals and birds have contracted melioidosis. Among 1420 soil samples collected from various sites in the oceanarium over a 15-month period, B. pseudomallei was detected in nine (0.6%) soil samples using bacterial culture, whereas it was detected in 96 (6.8%) soil samples using the specific PCR assay confirmed by sequencing. The PCR-positive samples were detected during various months, with higher detection rates observed during summer months. Positive PCR detection was significantly correlated with ambient temperature (P<0.0001) and relative humidity (P=0.011) but not with daily rainfall (P=0.241) or a recent typhoon (P=0.787). PCR-positive samples were obtained from all sampling locations, with the highest detection rate in the valley. Our results suggest that B. pseudomallei is prevalent and endemic in the oceanarium. The present PCR assay is more sensitive than the bacterial culture method, and it may be used to help better assess the transmission of melioidosis and to design infection control measures for captive animals in this unique and understudied environment.
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