ArticleThe Journal of biological chemistry2015
The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- New FLU mutant alleles point to new insights into singlet oxygen-mediated signal transduction.The New phytologist · 2026Article
- Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage.Molecular horticulture · 2026Article
- Singlet Oxygen Generation and Signaling in Higher Plants.International journal of molecular sciences · 2026Review
- Integrated GWAS and Transcriptome Analysis Reveal the Genetic and Molecular Basis of Low Nitrogen Tolerance in Maize Seedlings.Plants (Basel, Switzerland) · 2025Article
- Protective mechanisms of exogenous melatonin on chlorophyll metabolism and photosynthesis in tomato seedlings under heat stress.Frontiers in plant science · 2025Article
- A novel tetratricopeptide-repeat protein, TTP1, forms complexes with glutamyl-tRNA reductase and protochlorophyllide oxidoreductase during tetrapyrrole biosynthesis.Journal of experimental botany · 2024Article
- An alanine to valine mutation of glutamyl-tRNA reductase enhances 5-aminolevulinic acid synthesis in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Heme is involved in the exogenous ALA-promoted growth and antioxidant defense system of cucumber seedlings under salt stress.BMC plant biology · 2022Article
- Natural 5-Aminolevulinic Acid: Sources, Biosynthesis, Detection and Applications.Frontiers in bioengineering and biotechnology · 2022Review
- A biosynthetic pathway to aromatic amines that uses glycyl-tRNA as nitrogen donor.Nature chemistry · 2022Article
- Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi.BMC plant biology · 2021Article
- Identification and Functional Analysis of TomatoInternational journal of molecular sciences · 2021Article
- The Role of Membranes and Lipid-Protein Interactions in the Mg-Branch of Tetrapyrrole Biosynthesis.Frontiers in plant science · 2021Review
- Gene editing: an instrument for practical application of gene biology to plant breeding.Journal of Zhejiang University. Science. B · 2020Review
- The GluTR-binding protein is the heme-binding factor for feedback control of glutamyl-tRNA reductase.eLife · 2019Article
- Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation.Nature communications · 2018Article
- Crystal structure of Arabidopsis thaliana glutamyl-tRNAPhotosynthesis research · 2018Article
- 5-Aminolevulinic Acid (ALA) Alleviated Salinity Stress in Cucumber Seedlings by Enhancing Chlorophyll Synthesis Pathway.Frontiers in plant science · 2018Article
- LIL3, a Light-Harvesting Complex Protein, Links Terpenoid and Tetrapyrrole Biosynthesis inPlant physiology · 2017Article
- The catalytic subunit of magnesium-protoporphyrin IX monomethyl ester cyclase forms a chloroplast complex to regulate chlorophyll biosynthesis in rice.Plant molecular biology · 2016Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tetratricopeptide repeat (TPR)-containing protein FLU is a negative regulator of chlorophyll biosynthesis in plants. It directly interacts through its TPR domain with glutamyl-tRNA reductase (GluTR), the rate-limiting enzyme in the formation of δ-aminolevulinic acid (ALA). Delineation of how FLU binds to GluTR is important for understanding the molecular basis for FLU-mediated repression of synthesis of ALA, the universal tetrapyrrole precursor. Here, we characterize the FLU-GluTR interaction by solving the crystal structures of the uncomplexed TPR domain of FLU (FLU(TPR)) at 1.45-Å resolution and the complex of the dimeric domain of GluTR bound to FLU(TPR) at 2.4-Å resolution. Three non-canonical TPR motifs of each FLU(TPR) form a concave surface and clamp the helix bundle in the C-terminal dimeric domain of GluTR. We demonstrate that a 2:2 FLU(TPR)-GluTR complex is the functional unit for FLU-mediated GluTR regulation and suggest that the formation of the FLU-GluTR complex prevents glutamyl-tRNA, the GluTR substrate, from binding with this enzyme. These results also provide insights into the spatial regulation of ALA synthesis by the membrane-located FLU protein.
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