Evidence map›Paper›PMID 26037800›Full record

ArticleNeuroscience2015

An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction.

C R Hutch, C J Hillard, C Jia, C C Hegg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 18 citations in OpenAlex.

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  7. Cannabis Users Show Enhanced Expression of CBMolecular neurobiology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

C R HutchNeuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, USA; Environmental and Integrative Toxicological Sciences, Michigan State University, 1129 Farm Lane, East Lansing, MI 48824, USA. Electronic address: hutchche@umich.edu.
C J HillardDepartment of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA. Electronic address: chillard@mcw.edu.
C JiaDepartment of Pharmacology and Toxicology, Michigan State University, 1355 Bogue Street, East Lansing, MI 48824, USA. Electronic address: JIAC01@mail.etsu.edu.
C C HeggNeuroscience Program, Michigan State University, 293 Farm Lane, East Lansing, MI 48824, USA; Environmental and Integrative Toxicological Sciences, Michigan State University, 1129 Farm Lane, East Lansing, MI 48824, USA; Department of Pharmacology and Toxicology, Michigan State University, 1355 Bogue Street, East Lansing, MI 48824, USA. Electronic address: hegg@msu.edu.
Michigan State University · USMedical College of Wisconsin · US

Funding

Neurogenesis and chronic cannabinoid exposureR03DA033495 · NIDA · MICHIGAN STATE UNIVERSITY · PI HEGG, COLLEEN COSGROVE · 2013 to 2014
$154k
NIDA NIH HHS DA033495NIDA NIH HHS R03 DA033495
6 · The paper itself

Abstract

Endocannabinoids modulate a diverse array of functions including progenitor cell proliferation in the central nervous system, and odorant detection and food intake in the mammalian central olfactory system and larval Xenopus laevis peripheral olfactory system. However, the presence and role of endocannabinoids in the peripheral olfactory epithelium have not been examined in mammals. We found the presence of cannabinoid type 1 (CB1) and cannabinoid type 2 (CB2) receptor protein and mRNA in the olfactory epithelium. Using either immunohistochemistry or calcium imaging we localized CB1 receptors on neurons, glia-like sustentacular cells, microvillous cells and progenitor-like basal cells. To examine the role of endocannabinoids, CB1- and CB2- receptor-deficient (CB1(-/-)/CB2(-/-)) mice were used. The endocannabinoid 2-arachidonylglycerol (2-AG) was present at high levels in both C57BL/6 wildtype and CB1(-/-)/CB2(-/-) mice. 2-AG synthetic and degradative enzymes are expressed in wildtype mice. A small but significant decrease in basal cell and olfactory sensory neuron numbers was observed in CB1(-/-)/CB2(-/-) mice compared to wildtype mice. The decrease in olfactory sensory neurons did not translate to impairment in olfactory-mediated behaviors assessed by the buried food test and habituation/dishabituation test. Collectively, these data indicate the presence of an endocannabinoid system in the mouse olfactory epithelium. However, unlike in tadpoles, endocannabinoids do not modulate olfaction. Further investigation on the role of endocannabinoids in progenitor cell function in the olfactory epithelium is warranted.

Indexed as

AnimalsArachidonic AcidsEndocannabinoidsGlyceridesMaleMiceMice, Inbred C57BLMice, KnockoutOlfactory MucosaOlfactory Receptor NeuronsReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2RNA, MessengerSmellTissue Culture TechniquesArachidonic AcidsCNR1 protein, mouseCnr2 protein, mouseEndocannabinoidsGlyceridesglyceryl 2-arachidonateReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2RNA, Messenger2-AGcannabinoidsolfactionprogenitor cells

Identifiers

PMID26037800
PMCPMC4485596
OpenAlexW366076105

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.