ArticleRNA biology2015
MiR-2 family targets awd and fng to regulate wing morphogenesis in Bombyx mori.
Article in RNA biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Single-cell and spatial transcriptomics define 20E-driven developmental reprogramming in silkworm wing disc.Nature communications · 2026Article
- Insights into miRNAs of the Stingless BeeNon-coding RNA · 2025Article
- Developmental Expression Patterns of miRNA inGenes · 2025Article
- Molecular Characterization of miRNAs inBiology · 2024Article
- The lgi-miR-2d is Potentially Involved in Shell Melanin Synthesis by Targeting mitf in Manila Clam Ruditapes philippinarum.Marine biotechnology (New York, N.Y.) · 2024Article
- Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions.International journal of molecular sciences · 2023Review
- Identification and profiling of stable microRNAs in hemolymph of young and oldCurrent research in insect science · 2022Article
- Advances in Editing Silkworms (Insects · 2021Review
- Transcriptome dynamics during metamorphosis of imaginal discs into wings and thoracic dorsum in Apis mellifera castes.BMC genomics · 2021Article
- Elucidation of the MicroRNA Transcriptome in Western Corn Rootworm Reveals Its Dynamic and Evolutionary Complexity.Genomics, proteomics & bioinformatics · 2021Article
- The Ubiquitin Conjugating Enzyme UbcD1 is Required for Notch Signaling Activation DuringFrontiers in genetics · 2021Article
- Article
- Gtsf1 is essential for proper female sex determination and transposon silencing in the silkworm, Bombyx mori.PLoS genetics · 2020Article
- miR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein inRNA biology · 2020Article
- Post-transcriptional regulation of insect metamorphosis and oogenesis.Cellular and molecular life sciences : CMLS · 2020Review
- The miR-9b microRNA mediates dimorphism and development of wing in aphids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A fungal pathogen deploys a small silencing RNA that attenuates mosquito immunity and facilitates infection.Nature communications · 2019Article
- Comparative Identification of MicroRNAs inInsects · 2019Article
- CRISPR Disruption ofInsects · 2019Article
- Genome-Wide Analysis of MicroRNAs in Relation to Pupariation in Oriental Fruit Fly.Frontiers in physiology · 2019Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
MicroRNAs (miRNAs) are post-transcriptional regulators that target specific mRNAs for repression and thus play key roles in many biological processes, including insect wing morphogenesis. miR-2 is an invertebrate-specific miRNA family that has been predicted in the fruit fly, Drosophila melanogaster, to be involved in regulating the Notch signaling pathway. We show here that miR-2 plays a critical role in wing morphogenesis in the silkworm, Bombyx mori, a lepidopteran model insect. Transgenic over-expression of a miR-2 cluster using a Gal4/UAS system results in deformed adult wings, supporting the conclusion that miR-2 regulates functions essential for normal wing morphogenesis. Two genes, abnormal wing disc (awd) and fringe (fng), which are positive regulators in Notch signaling, are identified as miR-2 targets and validated by a dual-luciferase reporter assay. The relative abundance of both awd and fng expression products was reduced significantly in transgenic animals, implicating them in the abnormal wing phenotype. Furthermore, somatic mutagenesis analysis of awd and fng using the CRISPR/Cas9 system and knock-out mutants also resulted in deformed wings similar to those observed in the miR-2 overexpression transgenic animals. The critical role of miR-2 in Bombyx wing morphogenesis may provide a potential target in future lepidopteran pest control.
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