ArticlePlant physiology2015
Cellulose-Pectin Spatial Contacts Are Inherent to Never-Dried Arabidopsis Primary Cell Walls: Evidence from Solid-State Nuclear Magnetic Resonance.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 240 citations in OpenAlex.
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- Cell walls: a comparative view of the composition of cell surfaces of plants, algae, and microorganisms.Journal of experimental botany · 2025Review
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
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- The role of NMR spectroscopy in lignocellulosic biomass characterisation: A mini review.Food chemistry. Molecular sciences · 2024Article
- Xyloglucan deficiency leads to a reduction in turgor pressure and changes in cell wall properties, affecting early seedling establishment.Current biology : CB · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- A mutation in CsGME encoding GDP-mannose 3,5-epimerase results in little and wrinkled leaf in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Selective Detection of Intermediate-Amplitude Motion by Solid-State NMR.The journal of physical chemistry. B · 2024Article
- Sound waves alter the viability of tobacco cells via changes in cytosolic calcium, membrane integrity, and cell wall composition.PloS one · 2024Article
- Forces on and in the cell walls of living plants.Plant physiology · 2023Article
- Polysaccharide assemblies in fungal and plant cell walls explored by solid-state NMR.Structure (London, England : 1993) · 2023Review
- Cellulose assembles into helical bundles of uniform handedness in cell walls with abnormal pectin composition.The Plant journal : for cell and molecular biology · 2023Article
- Insights into cell wall changes during fruit softening from transgenic and naturally occurring mutants.Plant physiology · 2023Review
- Advances on the Study of Diurnal Flower-Opening Times of Rice.International journal of molecular sciences · 2023Review
- Slits in the wall! Pulvinar slits confer unique cell wall mechanics for leaf movement.Plant physiology · 2023Article
- Transcellular progression of infection threads in Medicago truncatula roots is associated with locally confined cell wall modifications.Current biology : CB · 2023Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The structural role of pectins in plant primary cell walls is not yet well understood because of the complex and disordered nature of the cell wall polymers. We recently introduced multidimensional solid-state nuclear magnetic resonance spectroscopy to characterize the spatial proximities of wall polysaccharides. The data showed extensive cross peaks between pectins and cellulose in the primary wall of Arabidopsis (Arabidopsis thaliana), indicating subnanometer contacts between the two polysaccharides. This result was unexpected because stable pectin-cellulose interactions are not predicted by in vitro binding assays and prevailing cell wall models. To investigate whether the spatial contacts that give rise to the cross peaks are artifacts of sample preparation, we now compare never-dried Arabidopsis primary walls with dehydrated and rehydrated samples. One-dimensional (13)C spectra, two-dimensional (13)C-(13)C correlation spectra, water-polysaccharide correlation spectra, and dynamics data all indicate that the structure, mobility, and intermolecular contacts of the polysaccharides are indistinguishable between never-dried and rehydrated walls. Moreover, a partially depectinated cell wall in which 40% of homogalacturonan is extracted retains cellulose-pectin cross peaks, indicating that the cellulose-pectin contacts are not due to molecular crowding. The cross peaks are observed both at -20 °C and at ambient temperature, thus ruling out freezing as a cause of spatial contacts. These results indicate that rhamnogalacturonan I and a portion of homogalacturonan have significant interactions with cellulose microfibrils in the native primary wall. This pectin-cellulose association may be formed during wall biosynthesis and may involve pectin entrapment in or between cellulose microfibrils, which cannot be mimicked by in vitro binding assays.
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