ArticlePloS one2015
Spatiotemporal stability of neonatal rat cardiomyocyte monolayers spontaneous activity is dependent on the culture substrate.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Development of a high-speed imaging system for real time evaluation and monitoring of cardiac engineered tissues.Frontiers in bioengineering and biotechnology · 2024Article
- Active force generation contributes to the complexity of spontaneous activity and to the response to stretch of murine cardiomyocyte cultures.The Journal of physiology · 2022Article
- Novel Analysis Method for Beating Cells Videomicroscopy Data: Functional Characterization of Culture Samples.Frontiers in physiology · 2022Article
- Dense optical flow software to quantify cellular contractility.Cell reports methods · 2021Article
- Muscular Thin Films for Label-Free Mapping of Excitation Propagation in Cardiac Tissue.Annals of biomedical engineering · 2020Article
- Cell Culture Platforms with Controllable Stiffness for Chick Embryonic Cardiomyocytes.Biomimetics (Basel, Switzerland) · 2019Article
- Self-organization of conducting pathways explains electrical wave propagation in cardiac tissues with high fraction of non-conducting cells.PLoS computational biology · 2019Article
- Mussel-inspired 3D fiber scaffolds for heart-on-a-chip toxicity studies of engineered nanomaterials.Analytical and bioanalytical chemistry · 2018Article
- Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.Scientific reports · 2018Article
- In silico study of multicellular automaticity of heterogeneous cardiac cell monolayers: Effects of automaticity strength and structural linear anisotropy.PLoS computational biology · 2018Article
- The Dome Wall of Bladder Acts as a Pacemaker Site in Detrusor Instability in Rats.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Modulation of the contractility of micropatterned myocardial cells with nanoscale forces using atomic force microscopy.NanobiomedicineArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In native conditions, cardiac cells must continuously comply with diverse stimuli necessitating a perpetual adaptation. Polydimethylsiloxane (PDMS) is commonly used in cell culture to study cellular response to changes in the mechanical environment. The aim of this study was to evaluate the impact of using PDMS substrates on the properties of spontaneous activity of cardiomyocyte monolayer cultures. We compared PDMS to the gold standard normally used in culture: a glass substrate. Although mean frequency of spontaneous activity remained unaltered, incidence of reentrant activity was significantly higher in samples cultured on glass compared to PDMS substrates. Higher spatial and temporal instability of the spontaneous rate activation was found when cardiomyocytes were cultured on PDMS, and correlated with decreased connexin-43 and increased CaV3.1 and HCN2 mRNA levels. Compared to cultures on glass, cultures on PDMS were associated with the strongest response to isoproterenol and acetylcholine. These results reveal the importance of carefully selecting the culture substrate for studies involving mechanical stimulation, especially for tissue engineering or pharmacological high-throughput screening of cardiac tissue analog.
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