ArticlePloS one2015
Conformational Changes in the Orai1 C-Terminus Evoked by STIM1 Binding.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Mechanistic insights into Orai dynamics during pore opening.Channels (Austin, Tex.) · 2026Review
- Water in peripheral TM-interfaces of Orai1-channels triggers pore opening.Communications biology · 2024Article
- Highlighting the Multifaceted Role of Orai1 N-Terminal- and Loop Regions for Proper CRAC Channel Functions.Cells · 2022Review
- Orai channel C-terminal peptides are key modulators of STIM-Orai coupling and calcium signal generation.Cell reports · 2021Article
- The Orai Pore Opening Mechanism.International journal of molecular sciences · 2021Review
- Molecular Choreography and Structure of CaMembranes · 2020Review
- The Intricate Coupling Between STIM Proteins and Orai Channels.Current opinion in physiology · 2020Article
- Molecular basis of allosteric Orai1 channel activation by STIM1.The Journal of physiology · 2020Review
- The remote allosteric control of Orai channel gating.PLoS biology · 2019Article
- Critical parameters maintaining authentic CRAC channel hallmarks.European biophysics journal : EBJ · 2019Review
- Toward a Model for Activation of Orai Channel.iScience · 2019Article
- ORAI1 channel gating and selectivity is differentially altered by natural mutations in the first or third transmembrane domain.The Journal of physiology · 2019Article
- STIM1 activation of Orai1.Cell calcium · 2019Review
- Stromal-Interacting Molecule 1 (Stim1)/Orai1 Modulates Endothelial Permeability in Ventilator-Induced Lung Injury.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- The exquisitely cooperative nature of Orai1 channel activation.The Journal of general physiology · 2018Article
- Physiological CRAC channel activation and pore properties require STIM1 binding to all six Orai1 subunits.The Journal of general physiology · 2018Article
- Article
- Pore properties of Orai1 calcium channel dimers and their activation by the STIM1 ER calcium sensor.The Journal of biological chemistry · 2018Article
- Mapping the functional anatomy of Orai1 transmembrane domains for CRAC channel gating.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Distinct gating mechanism of SOC channel involving STIM-Orai coupling and an intramolecular interaction of Orai inProceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Store-operated CRAC channels regulate a wide range of cellular functions including gene expression, chemotaxis, and proliferation. CRAC channels consist of two components: the Orai proteins (Orai1-3), which form the ion-selective pore, and STIM proteins (STIM1-2), which form the endoplasmic reticulum (ER) Ca2+ sensors. Activation of CRAC channels is initiated by the migration of STIM1 to the ER-plasma membrane (PM) junctions, where it directly interacts with Orai1 to open the Ca2+-selective pores of the CRAC channels. The recent elucidation of the Drosophila Orai structure revealed a hexameric channel wherein the C-terminal helices of adjacent Orai subunits associate in an anti-parallel orientation. This association is maintained by hydrophobic interactions between the Drosophila equivalents of human Orai1 residues L273 and L276. Here, we used mutagenesis and chemical cross-linking to assess the nature and extent of conformational changes in the self-associated Orai1 C-termini during STIM1 binding. We find that linking the anti-parallel coiled-coils of the adjacent Orai1 C-termini through disulfide cross-links diminishes STIM1-Orai1 interaction, as assessed by FRET. Conversely, prior binding of STIM1 to the Orai1 C-terminus impairs cross-linking of the Orai1 C-termini. Mutational analysis indicated that a bend of the Orai1 helix located upstream of the self-associated coils (formed by the amino acid sequence SHK) establishes an appropriate orientation of the Orai1 C-termini that is required for STIM1 binding. Together, our results support a model wherein the self-associated Orai1 C-termini rearrange modestly to accommodate STIM1 binding.
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