ArticlePloS one2015
Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 60 citations in OpenAlex.
- Secreted molecules as modulators of somatic embryogenesis efficiency - an overview.Frontiers in plant science · 2026Review
- Roles of reactive oxygen species, storage compounds, and endogenous hormones in regulating somatic embryogenesis in blue spruce (Frontiers in plant science · 2025Article
- An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Somatic Embryogenesis in Sugarcane (Saccharum spp.).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular Determinants ofFrontiers in plant science · 2022Review
- Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (× Triticosecale Wittm.) after 5-azacytidine treatment.Scientific reports · 2021Article
- Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants.International journal of molecular sciences · 2021Review
- Shotgun label-free proteomic and biochemical study of somatic embryos (cotyledonary and maturation stage) in3 Biotech · 2021Article
- Genes, proteins and other networks regulating somatic embryogenesis in plants.Journal, genetic engineering & biotechnology · 2020Review
- Full-Length Transcriptome Analysis of theInternational journal of molecular sciences · 2020Article
- Article
- Network Analyses and Data Integration of Proteomics and Metabolomics From Leaves of Two Contrasting Varieties of Sugarcane in Response to Drought.Frontiers in plant science · 2019Article
- iTRAQ-based comparative proteomic analysis provides insights into somatic embryogenesis in Gossypium hirsutum L.Plant molecular biology · 2018Article
- Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis.Frontiers in plant science · 2018Review
- Somatic Embryogenesis in Coffee: The Evolution of Biotechnology and the Integration of Omics Technologies Offer Great Opportunities.Frontiers in plant science · 2017Review
- Salt stress induces changes in the proteomic profile of micropropagated sugarcane shoots.PloS one · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to observe somatic embryo development and to identify differentially expressed proteins in embryogenic (E) and non-embryogenic (NE) callus during maturation treatment. E and NE callus were cultured on maturation culture medium supplemented with different concentrations (0.0, 0.75, 1.5 and 2.0 g L(-1)) of activated charcoal (AC). Somatic embryo formation and differential protein expression were evaluated at days 0 and 21 using shotgun proteomic analyses. Treatment with 1.5 g L(-1) AC resulted in higher somatic embryo maturation rates (158 somatic embryos in 14 days) in E callus but has no effect in NE callus. A total of 752 co-expressed proteins were identified through the SUCEST (The Sugarcane EST Project), including many housekeeping proteins. E callus showed 65 exclusive proteins on day 0, including dehydrogenase, desiccation-related protein, callose synthase 1 and nitric oxide synthase. After 21 days on maturation treatment, 14 exclusive proteins were identified in E callus, including catalase and secreted protein. NE callus showed 23 exclusive proteins on day 0 and 10 exclusive proteins after 21 days on maturation treatment, including many proteins related to protein degradation. The induction of maturation leads to somatic embryo development, which likely depends on the expression of specific proteins throughout the process, as seen in E callus under maturation treatment. On the other hand, some exclusive proteins can also specifically prevent of somatic embryos development, as seen in the NE callus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.