ArticleScientific reports2015
Decoupling the role of stiffness from other hydroxyapatite signalling cues in periosteal derived stem cell differentiation.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 53 citations in OpenAlex.
- The development of a collagen-nanoscale hydroxyapatite three-dimensional (3D) in vitro culture system for reproducing osteocyte differentiation and tissue mineralization.Biomaterials · 2026Article
- A periosteum-derived cell line to study the role of BMP/TGFβ signaling in periosteal cell behavior and function.Frontiers in physiology · 2023Article
- Decoupling the role of chemistry and microstructure in hMSCs response to an osteoinductive calcium phosphate ceramic.Bioactive materials · 2023Article
- Characterizing and Engineering Biomimetic Materials for Viscoelastic Mechanotransduction Studies.Tissue engineering. Part B, Reviews · 2022Review
- 3D Scaffolds to Model the Hematopoietic Stem Cell Niche: Applications and Perspectives.Materials (Basel, Switzerland) · 2021Review
- A Critical Review on the Design, Manufacturing and Assessment of the Bone Scaffold for Large Bone Defects.Frontiers in bioengineering and biotechnology · 2021Review
- Engineering Gels with Time-Evolving Viscoelasticity.Materials (Basel, Switzerland) · 2020Article
- Functionalized Enzyme-Responsive Biomaterials to Model Tissue StiffeningFrontiers in bioengineering and biotechnology · 2020Article
- Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization.Materials (Basel, Switzerland) · 2019Article
- Comparison of frequency and strain-rate domain mechanical characterization.Scientific reports · 2018Article
- Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions.Journal of visualized experiments : JoVE · 2018Article
- Bioinspired liver scaffold design criteria.Organogenesis · 2018Article
- Micro-Mechanical Viscoelastic Properties of Crosslinked Hydrogels Using the Nano-Epsilon Dot Method.Materials (Basel, Switzerland) · 2017Article
- Biofabrication and Bone Tissue Regeneration: Cell Source, Approaches, and Challenges.Frontiers in bioengineering and biotechnology · 2017Review
- On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids.PloS one · 2017Article
- Microenvironment complexity and matrix stiffness regulate breast cancer cell activity in a 3D in vitro model.Scientific reports · 2016Article
- In vitro osteogenic and odontogenic differentiation of human dental pulp stem cells seeded on carboxymethyl cellulose-hydroxyapatite hybrid hydrogel.Frontiers in physiology · 2015Article
- Stem cell origin differently affects bone tissue engineering strategies.Frontiers in physiology · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone extracellular matrix (ECM) is a natural composite made of collagen and mineral hydroxyapatite (HA). Dynamic cell-ECM interactions play a critical role in regulating cell differentiation and function. Understanding the principal ECM cues promoting osteogenic differentiation would be pivotal for both bone tissue engineering and regenerative medicine. Altering the mineral content generally modifies the stiffness as well as other physicochemical cues provided by composite materials, complicating the "cause-effect" analysis of resultant cell behaviour. To isolate the contribution of mechanical cues from other HA-derived signals, we developed and characterised composite HA/gelatin scaffolds with different mineral contents along with a set of stiffness-matched HA-free gelatin scaffolds. Samples were seeded with human periosteal derived progenitor cells (PDPCs) and cultured over 7 days, analysing their resultant morphology and gene expression. Our results show that both stiffness and HA contribute to directing PDPC osteogenic differentiation, highlighting the role of stiffness in triggering the expression of osteogenic genes and of HA in accelerating the process, particularly at high concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.