ArticleScientific reports2015
A comparative analysis of high-throughput platforms for validation of a circulating microRNA signature in diabetic retinopathy.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B-A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2024Pooled it
- Optimal endogenous controls for microRNA analysis of visceral adipose tissue in the NAFLD mouse model.Journal of biosciences · 2025Article
- Lessons and Applications of Omics Research in Diabetes Epidemiology.Current diabetes reports · 2024Review
- Identification of biomarkers associated with ferroptosis in diabetic retinopathy based on WGCNA and machine learning.Frontiers in genetics · 2024Article
- The link between glycemic control measures and eye microvascular complications in a clinical cohort of type 2 diabetes with microRNA-223-3p signature.Journal of translational medicine · 2023Article
- MicroRNA Profiling from Tears as a Potential Non-invasive Method for Early Detection of Diabetic Retinopathy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Viral Encoded miRNAs in Tumorigenesis: Theranostic Opportunities in Precision Oncology.Microorganisms · 2022Review
- Breast milk-derived extracellular vesicle miRNAs are associated with maternal asthma and atopy.Epigenomics · 2022Article
- A review of the emergence of antibiotic resistance in bioaerosols and its monitoring methods.Re/views in environmental science and bio/technology · 2022Review
- Decrease in Plasma miR-27a and miR-221 After Concussion in Australian Football Players.Biomarker insights · 2022Article
- A Cross-Comparison of High-Throughput Platforms for Circulating MicroRNA Quantification, Agreement in Risk Classification, and Biomarker Discovery in Non-Small Cell Lung Cancer.Frontiers in oncology · 2022Article
- Tear miRNA expression analysis reveals miR-203 as a potential regulator of corneal epithelial cells.BMC ophthalmology · 2021Article
- Postpartum circulating microRNA enhances prediction of future type 2 diabetes in women with previous gestational diabetes.Diabetologia · 2021Observational
- Description of a CSF-Enriched miRNA Panel for the Study of Neurological Diseases.Life (Basel, Switzerland) · 2021Article
- Insulin micro-secretion in Type 1 diabetes and related microRNA profiles.Scientific reports · 2021Article
- Machine learning workflows identify a microRNA signature of insulin transcription in human tissues.iScience · 2021Article
- Article
- The crucial choice of reference genes: identification of miR-191-5p for normalization of miRNAs expression in bone marrow mesenchymal stromal cell and HS27a/HS5 cell lines.Scientific reports · 2020Article
- Identification of a Plasma Microrna Signature as Biomarker of Subaneurysmal Aortic Dilation in Patients with High Cardiovascular Risk.Journal of clinical medicine · 2020Article
- Extracellular Vesicles and MicroRNA: Putative Role in Diagnosis and Treatment of Diabetic Retinopathy.Antioxidants (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs are now increasingly recognized as biomarkers of disease progression. Several quantitative real-time PCR (qPCR) platforms have been developed to determine the relative levels of microRNAs in biological fluids. We systematically compared the detection of cellular and circulating microRNA using a standard 96-well platform, a high-content microfluidics platform and two ultra-high content platforms. We used extensive analytical tools to compute inter- and intra-run variability and concordance measured using fidelity scoring, coefficient of variation and cluster analysis. We carried out unprejudiced next generation sequencing to identify a microRNA signature for Diabetic Retinopathy (DR) and systematically assessed the validation of this signature on clinical samples using each of the above four qPCR platforms. The results indicate that sensitivity to measure low copy number microRNAs is inversely related to qPCR reaction volume and that the choice of platform for microRNA biomarker validation should be made based on the abundance of miRNAs of interest.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.