ArticleBiopreservation and biobanking2015
Resilience of oocyte germinal vesicles to microwave-assisted drying in the domestic cat model.
Article in Biopreservation and biobanking, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- A 30-year journey in wildlife assisted reproductive technologies.Animal reproduction · 2026Review
- Dehydration-based preservation of cat cumulus-oocyte complexes is not improved by membrane-permeable trehalose.Frontiers in veterinary science · 2026Article
- Microwave-Assisted Dehydration, Non-Cryogenic Storage, and Rehydration of Spermatozoa and Germinal Vesicles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles†.Biology of reproduction · 2024Article
- The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes.Bioengineering (Basel, Switzerland) · 2023Article
- Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.Journal of assisted reproduction and genetics · 2023Article
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?Journal of assisted reproduction and genetics · 2022Article
- Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.Journal of assisted reproduction and genetics · 2022Article
- Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles.Cellular and molecular bioengineering · 2021Article
- Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1.Frontiers in cell and developmental biology · 2021Article
- Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model.BMC genomics · 2020Article
- Desiccation and supra-zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation.Molecular reproduction and development · 2019Article
- Article
- The mutual benefits of research in wild animal species and human-assisted reproduction.Journal of assisted reproduction and genetics · 2018Review
- Proteomic analysis of germinal vesicles in the domestic cat model reveals candidate nuclear proteins involved in oocyte competence acquisition.Molecular human reproduction · 2018Article
- Dry Preservation of Spermatozoa: Considerations for Different Species.Biopreservation and biobanking · 2017Review
- Synergistic Development of Biochips and Cell Preservation Methodologies: A Tale of Converging Technologies.Current stem cell reports · 2017Article
- Effects of Water on Structure and Dynamics of Trehalose Glasses at Low Water Contents and its Relationship to Preservation Outcomes.Scientific reports · 2016Article
- Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis.Journal of assisted reproduction and genetics · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The ability to compact and inject the cat germinal vesicle (GV) into a recipient cytoplast allows exploration of a new fertility preservation strategy that avoids whole oocyte freezing. The objective of the present study was to understand the impact of water loss and storage time on GV DNA integrity. Immature cat oocytes were exposed to 1.5 M trehalose for 10 min before microwave-assisted dehydration for 0, 5, 10, 15, 20, 25, 30, or 40 min. Oocytes then were rehydrated to assess chromatin configuration and the incidence of DNA fragmentation (TUNEL assay). The moisture content progressively decreased (p<0.05) from 1.7 to 0.1 gH2O/gDW over the first 30 min, but did not decrease further (p>0.05) after 40 min. Chromatin configuration was unaffected (p>0.05) over time. The percentage of GVs with DNA fragmentation was unaltered (p>0.05) from 0 to 30 min of treatment (range, 6.1%-12%), but increased (p<0.05) to 32.5% after 40 min. Next, the influence of storage at two different supra-zero temperatures after 30 min of drying was investigated. Oocyte-loaded, microwave-treated filters were individually sealed in Dri-Shield moisture barrier bags and stored at 4°C or ambient temperature for 0 to 8 weeks. Moisture contents gradually decreased (p<0.05) from 0.12 to 0.10 gH2O/gDW after 8 weeks of storage at 4°C or ambient temperature. The percentage of GVs with DNA fragmentation more than doubled (p<0.05) from 0 (14.3%) to 2 days (30.0%-33.0%), but remained stable (p>0.05) thereafter (1 through 4 weeks, 25.0%-35.0%). Collective results demonstrate the feasibility of using microwave processing to dehydrate the mammalian GV to a moisture content that is nonlethal and enables nonfrozen storage, an alternative approach for preserving the maternal genome at cool or ambient temperature.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.