Evidence map›Paper›PMID 26032781›Full record

ArticleDiagnostic pathology2015

Association between human papillomavirus and Epstein - Barr virus DNA and gene promoter methylation of RB1 and CDH1 in the cervical lesions: a transversal study.

Thaís M McCormick, Nathalie H S Canedo, Yara L Furtado, Filomena A Silveira, Roberto J de Lima, Andréa D F Rosman, Gutemberg L Almeida Filho, Maria da Glória da C Carvalho

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diagnostic pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 35 citations in OpenAlex.

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  7. [Influence of E-cadherin methylation on prognosis in children with acute lymphoblastic leukemia].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2023
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  19. Methylation pattern ofOncology letters · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Thaís M McCormickLaboratory of Molecular Pathology, Pathological Anatomy Service and Pathology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. maccormick.thais@gmail.com.
Nathalie H S CanedoLaboratory of Neuropathology, Pathological Anatomy Service and Pathology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. nathaliecanedo@gmail.com.
Yara L FurtadoGynecology Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. yarafurtadorj@terra.com.br.
Filomena A SilveiraGynecology Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. filomenasilveira@uol.com.br.
Roberto J de LimaGynecology Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. limarober@gmail.com.
Andréa D F RosmanGynecology Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. andrea.rosman@gmail.com.
Gutemberg L Almeida FilhoGynecology Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. gutemberg@gineco.ufrj.br.
Maria da Glória da C CarvalhoLaboratory of Molecular Pathology, Pathological Anatomy Service and Pathology Department, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, Brazil. gloria@gcarvalho.org.
Universidade Federal do Rio de Janeiro · BRHospital Universitário Clementino Fraga Filho · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman papillomavirus (HPV) inactivates the retinoblastoma 1 (RB1) gene by promoter methylation and reduces cellular E-cadherin expression by overexpression of DNA methyltransferase 1 (DNMT1). The Epstein-Barr virus (EBV) is an oncogenic virus that may be related to cervical carcinogenesis. In gastric cancer, it has been demonstrated that E-cadherin gene (CDH1) hypermethylation is associated with DNMT1 overexpression by EBV infection. Our aim was to analyze the gene promoter methylation frequency of RB1 and CDH1 and verify the association between that methylation frequency and HPV and EBV infection in cervical lesions.

methodsSixty-five samples were obtained from cervical specimens: 15 normal cervices, 17 low-grade squamous intraepithelial lesions (LSIL), 15 high-grade squamous intraepithelial lesions (HSIL), and 18 cervical cancers. HPV and EBV DNA testing was performed by PCR, and the methylation status was verified by MSP.

resultsHPV frequency was associated with cervical cancer cases (p = 0.005) but not EBV frequency (p = 0.732). Viral co-infection showed a statistically significant correlation with cancer (p = 0.027). No viral infection was detected in 33.3% (5/15) of controls. RB1 methylated status was associated with cancer (p = 0.009) and HPV infection (p = 0.042). CDH1 methylation was not associated with cancer (p = 0.181). Controls and LSIL samples did not show simultaneous methylation, while both genes were methylated in 27.8% (5/18) of cancer samples. In the presence of EBV, CDH1 methylation was present in 27.8% (5/18) of cancer samples. Only cancer cases presented RB1 promoter methylation in the presence of HPV and EBV (33.3%).

conclusionsThe methylation status of both genes increased with disease progression. With EBV, RB1 methylation was a tumor-associated event because only the cancer group presented methylated RB1 with HPV infection. HPV infection was shown to be significantly correlated with cancer conditions. The global methylation frequency was higher when HPV was present, showing its epigenetic role in cervical carcinogenesis. Nevertheless, EBV seems to be a cofactor and needs to be further investigated. VIRTUAL SLIDES: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1159157579149317 .

Indexed as

DNA MethylationPromoter Regions, GeneticAntigens, CDCadherinsCase-Control StudiesDisease ProgressionDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA, ViralEpigenesis, GeneticEpstein-Barr Virus InfectionsFemaleGene Expression Regulation, NeoplasticHerpesvirus 4, HumanHost-Pathogen InteractionsHuman Papillomavirus DNA TestsAntigens, CDCadherinsCDH1 protein, humanDNA (Cytosine-5-)-Methyltransferase 1DNA (Cytosine-5-)-MethyltransferasesDNA, ViralDNMT1 protein, humanRetinoblastoma Protein

Identifiers

PMID26032781
PMCPMC4450846
OpenAlexW1502708765

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.