ArticleMolecular ecology resources2016
Quantification of mesocosm fish and amphibian species diversity via environmental DNA metabarcoding.
Article in Molecular ecology resources, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed, 411 citations in OpenAlex.
- Unravelling Bulk Ichthyoplankton Diversity in Vietnam: Metabarcoding Validation With Controlled Mock Samples.Molecular ecology resources · 2026Article
- Spatial Complexity and Rare-Taxon Turnover Structures Benthic Coral Reef eDNA Communities.Molecular ecology · 2026Article
- Revealing seasonal dietary niche overlap among sympatric large carnivores using DNA metabarcoding.Scientific reports · 2026Article
- MitoNGS: an online platform to analyze fish metabarcoding data in high resolution.Molecular biology and evolution · 2026Article
- Environmental DNA (eDNA) Technology in Biodiversity and Ecosystem Health Research: Advances and Prospects.Ecology and evolution · 2026Review
- Surveying tropical faunal diversity via airborne DNA analyses.Scientific reports · 2025Article
- Spatiotemporal Patterns of Fish Diversity in the Waters Around the Five West Sea Islands of South Korea: Integrating Bottom Trawl and Environmental DNA (eDNA) Methods.Animals : an open access journal from MDPI · 2025Article
- Herbivory Dominates the Spring Diet of American Black Bears (Ecology and evolution · 2025Article
- Positive Correlation Between Economic Activities and Fish Diversity in Small River Basins of Less Developed Regions: A Case Study of the Lixian River Basin.Animals : an open access journal from MDPI · 2025Article
- Article
- Ecological Effects ofBiology · 2025Article
- Discriminating spatiotemporal heterogeneity and environmental drivers of fish assemblages using environmental DNA metabarcoding in mosaic habitat ecosystems.Scientific reports · 2025Article
- The Long and the Short of It: Nanopore-Based eDNA Metabarcoding of Marine Vertebrates Works; Sensitivity and Species-Level Assignment Depend on Amplicon Lengths.Molecular ecology resources · 2025Article
- Article
- Comparison of Universal mtDNA Primers in Species Identification of Animals in a Sample with Severely Degraded DNA.Animals : an open access journal from MDPI · 2024Article
- Multi-method survey rediscovers critically endangered species and strengthens Madagascar's freshwater fish conservation.Scientific reports · 2024Article
- Integrating invasive species risk assessment into environmental DNA metabarcoding reference libraries.Ecological applications : a publication of the Ecological Society of America · 2023Article
- Environmental DNA metabarcoding of water samples as a tool for monitoring Iberian freshwater fish composition.PloS one · 2023Article
- Assessing springtime vertebrate prey of sympatric mesopredators in the southeastern United States using metabarcoding analysis.PloS one · 2023Article
- Signal and noise in metabarcoding data.PloS one · 2023Article
37 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freshwater fauna are particularly sensitive to environmental change and disturbance. Management agencies frequently use fish and amphibian biodiversity as indicators of ecosystem health and a way to prioritize and assess management strategies. Traditional aquatic bioassessment that relies on capture of organisms via nets, traps and electrofishing gear typically has low detection probabilities for rare species and can injure individuals of protected species. Our objective was to determine whether environmental DNA (eDNA) sampling and metabarcoding analysis can be used to accurately measure species diversity in aquatic assemblages with differing structures. We manipulated the density and relative abundance of eight fish and one amphibian species in replicated 206-L mesocosms. Environmental DNA was filtered from water samples, and six mitochondrial gene fragments were Illumina-sequenced to measure species diversity in each mesocosm. Metabarcoding detected all nine species in all treatment replicates. Additionally, we found a modest, but positive relationship between species abundance and sequencing read abundance. Our results illustrate the potential for eDNA sampling and metabarcoding approaches to improve quantification of aquatic species diversity in natural environments and point the way towards using eDNA metabarcoding as an index of macrofaunal species abundance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.