ArticleBMC genomics2015
The complete methylome of Helicobacter pylori UM032.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Towards molecular evolutionary epigenomics with an expanded nucleotide code involving methylated bases.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
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- Methylome evolution suggests lineage-dependent selection in the gastric pathogen Helicobacter pylori.Communications biology · 2023Article
- Computational analysis of RNA methyltransferase Rv3366 as a potential drug target for combating drug-resistantFrontiers in molecular biosciences · 2023Article
- Biomarker Characterization and Prediction of Virulence and Antibiotic Resistance fromBiomolecules · 2022Review
- Methylation Motifs in Promoter Sequences May Contribute to the Maintenance of a ConservedMicroorganisms · 2021Article
- Comparison of long-read sequencing technologies in interrogating bacteria and fly genomes.G3 (Bethesda, Md.) · 2021Article
- Genome-wide identification of 5-methylcytosine sites in bacterial genomes by high-throughput sequencing of MspJI restriction fragments.PloS one · 2021Article
- Two DNA Methyltransferases for Site-Specific 6mA and 5mC DNA Modification inFrontiers in plant science · 2021Article
- Article
- A novel DNA methylation motif identified in Bacillus pumilus BA06 and possible roles in the regulation of gene expression.Applied microbiology and biotechnology · 2020Article
- Combined Methylome, Transcriptome and Proteome Analyses Document Rapid Acclimatization of a Bacterium to Environmental Changes.Frontiers in microbiology · 2020Article
- Networking and Specificity-Changing DNA Methyltransferases inFrontiers in microbiology · 2020Article
- Rational development of transformation in Clostridium thermocellum ATCC 27405 via complete methylome analysis and evasion of native restriction-modification systems.Journal of industrial microbiology & biotechnology · 2019Article
- Review of current diagnostic methods and advances inWorld journal of gastroenterology · 2019Review
- The core genome m5C methyltransferase JHP1050 (M.Hpy99III) plays an important role in orchestrating gene expression in Helicobacter pylori.Nucleic acids research · 2019Article
- Deciphering bacterial epigenomes using modern sequencing technologies.Nature reviews. Genetics · 2019Review
- The complete genome and methylome ofGut pathogens · 2019Article
- N6-Methyladenine DNA modification in Xanthomonas oryzae pv. oryzicola genome.Scientific reports · 2018Article
- The complete methylome of an entomopathogenic bacterium reveals the existence of loci with unmethylated Adenines.Scientific reports · 2018Article
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Authors and funding
14 authors.
Funding
Abstract
backgroundThe genome of the human gastric pathogen Helicobacter pylori encodes a large number of DNA methyltransferases (MTases), some of which are shared among many strains, and others of which are unique to a given strain. The MTases have potential roles in the survival of the bacterium. In this study, we sequenced a Malaysian H. pylori clinical strain, designated UM032, by using a combination of PacBio Single Molecule, Real-Time (SMRT) and Illumina MiSeq next generation sequencing platforms, and used the SMRT data to characterize the set of methylated bases (the methylome).
resultsThe N4-methylcytosine and N6-methyladenine modifications detected at single-base resolution using SMRT technology revealed 17 methylated sequence motifs corresponding to one Type I and 16 Type II restriction-modification (R-M) systems. Previously unassigned methylation motifs were now assigned to their respective MTases-coding genes. Furthermore, one gene that appears to be inactive in the H. pylori UM032 genome during normal growth was characterized by cloning.
conclusionConsistent with previously-studied H. pylori strains, we show that strain UM032 contains a relatively large number of R-M systems, including some MTase activities with novel specificities. Additional studies are underway to further elucidating the biological significance of the R-M systems in the physiology and pathogenesis of H. pylori.
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