ArticleMolecular microbiology2015
TcPho91 is a contractile vacuole phosphate sodium symporter that regulates phosphate and polyphosphate metabolism in Trypanosoma cruzi.
Article in Molecular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- Outlining the structure of cAMP compartments in Trypanosoma cruzi.Trends in parasitology · 2025Review
- The adhesion plaque mediates fluid discharge and duplication of the contractile vacuole complex in Trypanosoma cruzi.Journal of cell science · 2025Article
- Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes.Microbiology and molecular biology reviews : MMBR · 2024Review
- Structure and dynamics of the contractile vacuole complex in Tetrahymena thermophila.Journal of cell science · 2023Article
- Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- Signaling pathways involved in environmental sensing in Trypanosoma cruzi.Molecular microbiology · 2021Review
- Xenotropic and polytropic retrovirus receptor 1 regulates procoagulant platelet polyphosphate.Blood · 2021Article
- A single Na+-Pi cotransporter in Toxoplasma plays key roles in phosphate import and control of parasite osmoregulation.PLoS pathogens · 2020Article
- A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression inFrontiers in cellular and infection microbiology · 2020Article
- State-of-the-art CRISPR/Cas9 Technology for Genome Editing in Trypanosomatids.The Journal of eukaryotic microbiology · 2019Review
- A Novel Calcium-Activated Potassium Channel Controls Membrane Potential and Intracellular pH inFrontiers in cellular and infection microbiology · 2019Article
- 5-Diphosphoinositol pentakisphosphate (5-IPThe Journal of biological chemistry · 2018Article
- HJournal of bioenergetics and biomembranes · 2017Article
- Polyphosphate and acidocalcisomes.Biochemical Society transactions · 2016Review
- The origin and evolution of the acidocalcisome and its interactions with other organelles.Molecular and biochemical parasitologyReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have identified a phosphate transporter (TcPho91) localized to the bladder of the contractile vacuole complex (CVC) of Trypanosoma cruzi, the etiologic agent of Chagas disease. TcPho91 has 12 transmembrane domains, an N-terminal regulatory SPX (named after SYG1, Pho81 and XPR1) domain and an anion permease domain. Functional expression in Xenopus laevis oocytes followed by two-electrode voltage clamp showed that TcPho91 is a low-affinity transporter with a Km for Pi in the millimolar range, and sodium-dependency. Epimastigotes overexpressing TcPho91-green fluorescent protein have significantly higher levels of pyrophosphate (PPi ) and short-chain polyphosphate (polyP), suggesting accumulation of Pi in these cells. Moreover, when overexpressing parasites were maintained in a medium with low Pi , they grew at higher rates than control parasites. Only one allele of TcPho91 in the CL strain encodes for the complete open reading frame, while the other one is truncated encoding for only the N-terminal domain. Taking advantage of this characteristic, knockdown experiments were performed resulting in cells with reduced growth rate as well as a reduction in PPi and short-chain polyP levels. Our results indicate that TcPho91 is a phosphate sodium symporter involved in Pi homeostasis in T. cruzi.
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