ArticlePloS one2015
Sequencing and De Novo Assembly of the Gonadal Transcriptome of the Endangered Chinese Sturgeon (Acipenser sinensis).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 91 citations in OpenAlex.
- De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Mature Male and Female Gonads inAnimals : an open access journal from MDPI · 2025Article
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- Identification of ActivinβA and Gonadotropin Regulation of the Activin System in the Ovary of Chinese SturgeonAnimals : an open access journal from MDPI · 2024Article
- Comparable to 17α- methyl testosterone, dietary supplements of Tribulus terrestris and Mucuna pruriens promote the development of mono-sex, all-male tilapia fry, growth, survival rate and sex-related genes (Amh, Sox9, Foxl2, Dmrt1).BMC veterinary research · 2024Article
- Transcriptome sequencing of the endangered land snail Karaftohelix adamsi from the Island Ulleung: De novo assembly, annotation, valuation of fitness genes and SSR markers.Genes & genomics · 2024Article
- Article
- Transcript annotation of Chinese sturgeon (Acipenser sinensis) using Iso-seq and RNA-seq data.Scientific data · 2023Article
- Developmental RNA-Seq transcriptomics of haploid germ cells and spermatozoa uncovers novel pathways associated with teleost spermiogenesis.Scientific reports · 2022Article
- Comparison of differential expression genes in ovaries and testes of Pearlscale angelfish Centropyge vrolikii based on RNA-Seq analysis.Fish physiology and biochemistry · 2021Article
- De Novo Transcriptomic Characterization Enables Novel Microsatellite Identification and Marker Development inLife (Basel, Switzerland) · 2021Article
- Differential expression analysis and identification of sex-related genes by gonad transcriptome sequencing in estradiol-treated and non-treated Ussuri catfish Pseudobagrus ussuriensis.Fish physiology and biochemistry · 2021Article
- Article
- Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Frontiers in cell and developmental biology · 2021Article
- Serum amyloid A is a positive acute phase protein in Russian sturgeon challenged with Aeromonas hydrophila.Scientific reports · 2020Article
- Transcriptome profiles of sturgeon lateral line electroreceptor and mechanoreceptor during regeneration.BMC genomics · 2020Article
- Siberian sturgeon multi-tissue reference transcriptome database.Database : the journal of biological databases and curation · 2020Article
- Genome survey and development of polymorphic microsatellite loci for Sillago sihama based on Illumina sequencing technology.Molecular biology reports · 2020Article
- Article
- Full-length transcriptome sequencing and comparative transcriptomic analysis to uncover genes involved in early gametogenesis in the gonads of Amur sturgeon (Frontiers in zoology · 2020Article
- Hypothalamus-pituitary-gonad axis transcriptome profiling for sex differentiation in Acipenser sinensis.Scientific data · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Chinese sturgeon (Acipenser sinensis) is endangered through anthropogenic activities including over-fishing, damming, shipping, and pollution. Controlled reproduction has been adopted and successfully conducted for conservation. However, little information is available on the reproductive regulation of the species. In this study, we conducted de novo transcriptome assembly of the gonad tissue to create a comprehensive dataset for A. sinensis.
resultsThe Illumina sequencing platform was adopted to obtain 47,333,701 and 47,229,705 high quality reads from testis and ovary cDNA libraries generated from three-year-old A. sinensis. We identified 86,027 unigenes of which 30,268 were annotated in the NCBI non-redundant protein database and 28,281 were annotated in the Swiss-prot database. Among the annotated unigenes, 26,152 and 7,734 unigenes, respectively, were assigned to gene ontology categories and clusters of orthologous groups. In addition, 12,557 unigenes were mapped to 231 pathways in the Kyoto Encyclopedia of Genes and Genomes Pathway database. A total of 1,896 unigenes, potentially differentially expressed between the two gonad types, were found, with 1,894 predicted to be up-regulated in ovary and only two in testis. Fifty-five potential gametogenesis-related genes were screened in the transcriptome and 34 genes with significant matches were found. Besides, more paralogs of 11 genes in three gene families (sox, apolipoprotein and cyclin) were found in A. sinensis compared to their orthologs in the diploid Danio rerio. In addition, 12,151 putative simple sequence repeats (SSRs) were detected.
conclusionsThis study provides the first de novo transcriptome analysis currently available for A. sinensis. The transcriptomic data represents the fundamental resource for future research on the mechanism of early gametogenesis in sturgeons. The SSRs identified in this work will be valuable for assessment of genetic diversity of wild fish and genealogy management of cultured fish.
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