ArticleScientific reports2015
Placement of oppositely charged aminoacids at a polypeptide termini determines the voltage-controlled braking of polymer transport through nanometer-scale pores.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Bioinformatic and computational biophysics tools for nanopore engineering: a review from standard approaches to machine learning advancements.Journal of nanobiotechnology · 2026Review
- Complex and Non-sequential Current Signatures of a β-Hairpin Peptide Confined in a Nanopore.Analytical chemistry · 2025Article
- Controlling DNA Fragments Translocation across Nanopores with the Synergic Use of Site-Directed Mutagenesis, pH-Dependent Charge Tuning, and Electroosmotic Flow.ACS applied materials & interfaces · 2024Article
- Review
- Nanopore discrimination and sensitive plasma detection of multiple natriuretic peptides: The representative biomarker of human heart failure.Biosensors & bioelectronics · 2023Article
- Aerolysin nanopore-based identification of proteinogenic amino acids using a bipolar peptide probe.Nanoscale advances · 2022Article
- Nanopore sensing: A physical-chemical approach.Biochimica et biophysica acta. Biomembranes · 2021Review
- Article
- Multiplex quantitative detection of SARS-CoV-2 specific IgG and IgM antibodies based on DNA-assisted nanopore sensing.Biosensors & bioelectronics · 2021Article
- The emerging landscape of single-molecule protein sequencing technologies.Nature methods · 2021Review
- The Nanopore-Tweezing-Based, Targeted Detection of Nucleobases on Short Functionalized Peptide Nucleic Acid Sequences.Polymers · 2021Article
- Analytical Model for Particle Capture in Nanopores Elucidates Competition among Electrophoresis, Electroosmosis, and Dielectrophoresis.ACS nano · 2020Article
- N-Terminal Derivatization-Assisted Identification of Individual Amino Acids Using a Biological Nanopore Sensor.ACS sensors · 2020Article
- Correction to Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological Nanopore.ACS nano · 2020Article
- Application of Solid-State Nanopore in Protein Detection.International journal of molecular sciences · 2020Review
- Different Anomeric Sugar Bound States of Maltose Binding Protein Resolved by a Cytolysin A Nanopore Tweezer.ACS nano · 2020Article
- Single-molecule Study on the Interactions between Cyclic Nonribosomal Peptides and Protein Nanopore.ACS applied bio materials · 2020Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Insights into protein sequencing with an α-Hemolysin nanopore by atomistic simulations.Scientific reports · 2019Article
- Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Protein and solid-state nanometer-scale pores are being developed for the detection, analysis, and manipulation of single molecules. In the simplest embodiment, the entry of a molecule into a nanopore causes a reduction in the latter's ionic conductance. The ionic current blockade depth and residence time have been shown to provide detailed information on the size, adsorbed charge, and other properties of molecules. Here we describe the use of the nanopore formed by Staphylococcus aureus α-hemolysin and polypeptides with oppositely charged segments at the N- and C-termini to increase both the polypeptide capture rate and mean residence time of them in the pore, regardless of the polarity of the applied electrostatic potential. The technique provides the means to improve the signal to noise of single molecule nanopore-based measurements.
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