Evidence map›Paper›PMID 26029729›Full record

ArticleMolecular therapy. Methods & clinical development2015

Ototoxicity-induced loss of hearing and inner hair cells is attenuated by HSP70 gene transfer.

Yohei Takada, Tomoko Takada, Min Young Lee, Donald L Swiderski, Lisa L Kabara, David F Dolan, Yehoash Raphael

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Methods & clinical development, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Review
  3. Advancing precision ear medicine: leveraging animal models for disease insights and therapeutic innovations.Mammalian genome : official journal of the International Mammalian Genome Society · 2025
    Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Genetic Hearing Loss and Gene Therapy.Genomics & informatics · 2018
    Article
  10. Emerging Gene Therapies for Genetic Hearing Loss.Journal of the Association for Research in Otolaryngology : JARO · 2017
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yohei TakadaDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
Tomoko TakadaDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
Min Young LeeDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
Donald L SwiderskiDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
Lisa L KabaraDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
David F DolanDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.
Yehoash RaphaelDepartment of Otolaryngology-Head and Neck Surgery, Kresge Hearing Research Institute, University of Michigan , Ann Arbor, Michigan, USA.

Funding

Scientific Synergy CoreP30DC005188 · NIDCD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DOLAN, DAVID FRANCIS · 2002 to 2016
$10.3M
Cochlear Implants in Reinnervated EarsR01DC010412 · NIDCD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI RAPHAEL, YEHOASH · 2010 to 2014
$2.2M
NIDCD NIH HHS P30 DC005188NIDCD NIH HHS R01 DC010412
6 · The paper itself

Abstract

The most common reason for sensorineural deafness is death of hair cells (HCs). Heat shock proteins (HSPs) are molecular chaperones that participate in folding, targeting, and degrading proteins. HSP expression is increased in response to various environmental stresses to protect cells from damage. Here, we tested whether viral-mediated overexpression of HSP70 can protect HCs and hearing from severe ototoxicity (kanamycin and furosemide) in guinea pigs. Adenovirus-HSP70 mCherry (Ad.HSP70-mCherry) was injected to experimental animals and adenovirus-mCherry to controls, 4 days before the ototoxic insult. Hearing thresholds were measured by auditory brainstem response before the insult and again before sacrificing the animals, 14 days after the insult. Epi-fluorescence immunocytochemistry showed that injection of Ad.HSP70-mCherry resulted in mCherry fluorescence in nonsensory cells of the organ of Corti. The ototoxic insult eliminated both outer HCs and inner HCs throughout most of the cochlea of control (adenovirus-mCherry-injected) ears and contralateral (uninjected) ears. Ad.HSP70-mCherry-injected ears exhibited a significant preservation of inner HCs compared to control and contralateral ears, but outer HCs were not protected. Auditory brainstem response thresholds were significantly better in Ad.HSP70-mCherry-injected ears than in control and contralateral ears. Our data show that HSP70 augmentation may represent a potential therapy attenuating ototoxic inner HC loss.

Identifiers

PMID26029729
PMCPMC4445521

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.