ArticleRice (New York, N.Y.)2015
Transcriptome profiling of the spl5 mutant reveals that SPL5 has a negative role in the biosynthesis of serotonin for rice disease resistance.
Article in Rice (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- CDP-DAG synthases regulate plant growth and broad-spectrum disease resistance.Plant signaling & behavior · 2025Article
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- Integrative transcriptome and metabolome evaluation of melanin biosynthesis inForestry research · 2025Article
- Lesion mimic geneFrontiers in plant science · 2025Article
- Alternative polyadenylation profiles of susceptible and resistant rice (Oryza sativa L.) in response to bacterial leaf blight using RNA-seq.BMC plant biology · 2024Article
- Transcriptome analysis of auxin transcription factor OsARF17-mediated rice stripe mosaic virus response in rice.Frontiers in microbiology · 2023Article
- Advances in the Genetic Basis and Molecular Mechanism of Lesion Mimic Formation in Rice.Plants (Basel, Switzerland) · 2022Review
- Rice Lesion Mimic Gene Cloning and Association Analysis for Disease Resistance.Current issues in molecular biology · 2022Review
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- Identification and fine mapping of lesion mimic mutantBreeding science · 2021Article
- Enhanced SA and CaMolecular genetics and genomics : MGG · 2021Article
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- HbWRKY27, a group IIe WRKY transcription factor, positively regulates HbFPS1 expression in Hevea brasiliensis.Scientific reports · 2020Article
- De novo assembly of the Platycladus orientalis (L.) Franco transcriptome provides insight into the development and pollination mechanism of female cone based on RNA-Seq data.Scientific reports · 2019Article
- OsACL-A2 negatively regulates cell death and disease resistance in rice.Plant biotechnology journal · 2019Article
- Functional inactivation of OsGCNT induces enhanced disease resistance to Xanthomonas oryzae pv. oryzae in rice.BMC plant biology · 2018Article
- Metabolome Analysis of Multi-Connected Biparental Chromosome Segment Substitution Line Populations.Plant physiology · 2018Article
- The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice.PLoS genetics · 2016Article
- Ascribing Functions to Genes: Journey Towards Genetic Improvement of Rice Via Functional Genomics.Current genomics · 2016Article
- Phenolic Phytoalexins in Rice: Biological Functions and Biosynthesis.International journal of molecular sciences · 2015Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRice mutant, spl5 (spotted leaf 5), has spontaneous hypersensitive-like lesions on its leaves and shows enhanced resistance to pathogens, indicating that SPL5 plays a role in programmed cell death (PCD) and disease resistance. To understand the molecular mechanism of SPL5 gene, we investigated the transcriptome profiles of the spl5 mutant leaves with few lesions (FL) and leaves with many lesions (ML) compared to the wild-type (WT) leaves respectively by microarray.
resultsThe data from microarray revealed that 243 and 896 candidate genes (Fold change ≥ 3.0) were up- or down-regulated in the spl5-FL and spl5-ML, respectively, and a large number of these genes involved in biotic defense responses or reactive oxygen species (ROS) metabolism. Interestingly, according to our microarray and real-time PCR assays, the expressions of a transcription factor OsWRKY14 and genes responsible for the biosynthesis of serotonin, anthranilate synthase (AS), indole-3-glycerolphosphate synthase (IGPS), tryptophan synthase (TS) and tryptophan decarboxylase (TDC) were significantly up-regulated in the spl5 mutant. It has been reported previously that TS and TDC expressions are regulated by OsWRKY14 in rice, which raises the possibility that OsWRKY14 regulates serotonin production through the up-regulation of TS and TDC. Our HPLC analysis further confirmed that serotonin levels were higher in the leaves of spl5 mutant than that in WT.
conclusionsSince the serotonin plays a critical role in inducing disease-resistance, the increased serotonin level may contribute, at least partly, to the disease resistance in spl5. The SPL5 gene may act as a negative regulatory factor activating the serotonin metabolic pathway, and these results might provide a new insight into the spl5-induced defense response mechanisms in plants.
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