ArticleFrontiers in microbiology2015
Segregation of chromosome arms in growing and non-growing Escherichia coli cells.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- The role of active mRNA-ribosome dynamics and closing constriction in daughter chromosome separation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Relative Distribution of DnaA and DNA inInternational journal of molecular sciences · 2025Article
- Dynamic transitions of initiator binding coordinate the replication of the two chromosomes in Vibrio cholerae.Nature communications · 2025Article
- Compaction and Segregation of DNA inLife (Basel, Switzerland) · 2024Review
- Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli.Nature communications · 2023Article
- Engineering Tissue-Scale Properties with Synthetic Cells: Forging One from Many.ACS synthetic biology · 2023Review
- The Bacterial Nucleoid: From Electron Microscopy to Polymer Physics-A Personal Recollection.Life (Basel, Switzerland) · 2023Review
- Coupling between DNA replication, segregation, and the onset of constriction in Escherichia coli.Cell reports · 2022Article
- Stochastic nucleoid segregation dynamics as a source of the phenotypic variability in E. coli.Biophysical journal · 2021Article
- Towards a synthetic cell cycle.Nature communications · 2021Review
- Direct observation of independently moving replisomes in Escherichia coli.Nature communications · 2020Article
- Cell Boundary Confinement Sets the Size and Position of the E. coli Chromosome.Current biology : CB · 2019Article
- Different Amounts of DNA in Newborn Cells ofFrontiers in microbiology · 2018Article
- Kinetics of large-scale chromosomal movement during asymmetric cell division in Escherichia coli.PLoS genetics · 2017Article
- The role of MatP, ZapA and ZapB in chromosomal organization and dynamics in Escherichia coli.Nucleic acids research · 2016Article
- Chromosome replication, cell growth, division and shape: a personal perspective.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In slow-growing Escherichia coli cells the chromosome is organized with its left (L) and right (R) arms lying separated in opposite halves of the nucleoid and with the origin (O) in-between, giving the pattern L-O-R. During replication one of the arms has to pass the other to obtain the same organization in the daughter cells: L-O-R L-O-R. To determine the movement of arms during segregation six strains were constructed carrying three colored loci: the left and right arms were labeled with red and cyan fluorescent-proteins, respectively, on loci symmetrically positioned at different distances from the central origin, which was labeled with green-fluorescent protein. In non-replicating cells with the predominant spot pattern L-O-R, initiation of replication first resulted in a L-O-O-R pattern, soon changing to O-L-R-O. After replication of the arms the predominant spot patterns were, L-O-R L-O-R, O-R-L R-O-L or O-L-R L-O-R indicating that one or both arms passed an origin and the other arm. To study the driving force for these movements cell growth was inhibited with rifampicin allowing run-off DNA synthesis. Similar spot patterns were obtained in growing and non-growing cells, indicating that the movement of arms is not a growth-sustained process, but may result from DNA synthesis itself. The distances between loci on different arms (LR-distances) and between duplicated loci (LL- or RR-distances) as a function of their distance from the origin, indicate that in slow-growing cells DNA is organized according to the so-called sausage model and not according to the doughnut model.
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