ArticleStem cell reports2015
Efficient Generation of Cardiac Purkinje Cells from ESCs by Activating cAMP Signaling.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Towards chamber specific heart-on-a-chip for drug testing applications.Advanced drug delivery reviews · 2020Pooled it
- High sucralose exposure in early pregnancy increases the susceptibility of heart defects in mice offspring.iScience · 2026Article
- Transient Notch Activation Converts Pluripotent Stem Cell-Derived Cardiomyocytes Towards a Purkinje Fiber Fate.bioRxiv : the preprint server for biology · 2024Article
- Modulation of cAMP/cGMP signaling as prevention of congenital heart defects in Pde2A deficient embryos: a matter of oxidative stress.Cell death & disease · 2024Article
- Heart rhythmFrontiers in cardiovascular medicine · 2024Article
- Knockout of the Cardiac Transcription Factor NKX2-5 Results in Stem Cell-Derived Cardiac Cells with Typical Purkinje Cell-like Signal Transduction and Extracellular Matrix Formation.International journal of molecular sciences · 2023Article
- Article
- Application of Three-Dimensional Culture Method in the Cardiac Conduction System Research.Methods and protocols · 2022Article
- Modeling Human Heart Development and Congenital Defects Using Organoids: How Close Are We?Journal of cardiovascular development and disease · 2022Review
- Enhancing Matured Stem-Cardiac Cell Generation and Transplantation: A Novel Strategy for Heart Failure Therapy.Journal of cardiovascular translational research · 2021Review
- An experimental study on a piezoelectric vibration energy harvester for self-powered cardiac pacemakers.Annals of translational medicine · 2021Article
- Phenotypic technologies in stem cell biology.Cell chemical biology · 2021Review
- Uniformly-aligned gelatin/polycaprolactone fibers promote proliferation in adipose-derived stem cells and have distinct effects on cardiac cell differentiation.International journal of clinical and experimental pathology · 2021Article
- hiPSC-Derived Cardiac Tissue for Disease Modeling and Drug Discovery.International journal of molecular sciences · 2020Review
- 3D bioprinting and its potential impact on cardiac failure treatment: An industry perspective.APL bioengineering · 2020Article
- TRANSCRIPTIONAL REGULATION OF THE CARDIAC CONDUCTION SYSTEM.Transactions of the American Clinical and Climatological Association · 2020Article
- Activation-Induced Cytidine Deaminase Regulates Fibroblast Growth Factor/Extracellular Signal-Regulated Kinases Signaling to Achieve the Naïve Pluripotent State During Reprogramming.Stem cells (Dayton, Ohio) · 2019Article
- Changes in the spatial distribution of the Purkinje network after acute myocardial infarction in the pig.PloS one · 2019Article
- Screening-Based Chemical Approaches to Unravel Stem Cell Biology.Stem cell reports · 2018Review
- Transcriptional regulation of the cardiac conduction system.Nature reviews. Cardiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Dysfunction of the specialized cardiac conduction system (CCS) is associated with life-threatening arrhythmias. Strategies to derive CCS cells, including rare Purkinje cells (PCs), would facilitate models for mechanistic studies and drug discovery and also provide new cellular materials for regenerative therapies. A high-throughput chemical screen using CCS:lacz and Contactin2:egfp (Cntn2:egfp) reporter embryonic stem cell (ESC) lines was used to discover a small molecule, sodium nitroprusside (SN), that efficiently promotes the generation of cardiac cells that express gene profiles and generate action potentials of PC-like cells. Imaging and mechanistic studies suggest that SN promotes the generation of PCs from cardiac progenitors initially expressing cardiac myosin heavy chain and that it does so by activating cyclic AMP signaling. These findings provide a strategy to derive scalable PCs, along with insight into the ontogeny of CCS development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.