ArticleNano letters2015
Efficient Production of Single-Stranded Phage DNA as Scaffolds for DNA Origami.
Article in Nano letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed.
- Alternating Shear Force of Respiration Regulates Cell Interactions of Fibroblasts and Macrophages to Promote Soft Tissue Integration of Chest Wall Polyetheretherketone Implants.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Prospects of DNA nanotechnology in stroke repair and regeneration.Communications biology · 2026Review
- Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures.Materials today. Bio · 2026Article
- Complex cooperativity in DNA origami revealed via design-dependent defectivity.Nucleic acids research · 2026Article
- DNA Origami-Templated Aptamer Chiral Structures Realize Cellular Enantioselectivity.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Engineering Novel DNA Nanoarchitectures for Targeted Drug Delivery and Aptamer mediated Apoptosis in Cancer Therapeutics.Advanced functional materials · 2025Article
- Engineering a custom-sized DNA scaffold for more efficient DNA origami-based nucleic acid data storage.Synthetic biology (Oxford, England) · 2025Article
- Plugging synthetic DNA nanoparticles into the central dogma of life.Chemical communications (Cambridge, England) · 2024Review
- Quantification of ssDNA Scaffold Production by Ion-Pair Reverse Phase Chromatography.ACS omega · 2024Article
- Hepatitis B Virus Neutralization with DNA Origami Nanoshells.ACS applied materials & interfaces · 2024Article
- Genetically engineered bacteriophages as novel nanomaterials: applications beyond antimicrobial agents.Frontiers in bioengineering and biotechnology · 2024Review
- Customized Scaffolds for Direct Assembly of Functionalized DNA Origami.ACS applied materials & interfaces · 2023Article
- Triple-Stranded DNA As a Structural Element in DNA Origami.ACS nano · 2023Article
- Nucleic acid nanostructures forApplied physics reviews · 2023Review
- Design, Assembly, and Function of DNA Origami Mechanisms.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Energy landscapes of rotary DNA origami devices determined by fluorescence particle tracking.Biophysical journal · 2022Article
- Synthesizing the biochemical and semiconductor worlds:Nanoscale · 2022Review
- A DNA origami rotary ratchet motor.Nature · 2022Article
- Designer DNA nanostructures for viral inhibition.Nature protocols · 2022Review
- Antigen-Triggered Logic-Gating of DNA Nanodevices.Journal of the American Chemical Society · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Scaffolded DNA origami enables the fabrication of a variety of complex nanostructures that promise utility in diverse fields of application, ranging from biosensing over advanced therapeutics to metamaterials. The broad applicability of DNA origami as a material beyond the level of proof-of-concept studies critically depends, among other factors, on the availability of large amounts of pure single-stranded scaffold DNA. Here, we present a method for the efficient production of M13 bacteriophage-derived genomic DNA using high-cell-density fermentation of Escherichia coli in stirred-tank bioreactors. We achieve phage titers of up to 1.6 × 10(14) plaque-forming units per mL. Downstream processing yields up to 410 mg of high-quality single-stranded DNA per one liter reaction volume, thus upgrading DNA origami-based nanotechnology from the milligram to the gram scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.