ArticleNature communications2015
Direct observation of TALE protein dynamics reveals a two-state search mechanism.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Redesign of TALE proteins for DNA-templated assembly of protein fibers.Nature communications · 2026Article
- Effector Triggered Susceptibility and Immunity in Bacterial Diseases of Leaf Blight and Streak in Rice.Rice (New York, N.Y.) · 2026Review
- SMITracker: An Interactive Platform for Tracking and Analysis of Single-Molecule Interactions with Linear Substrates.Computational and structural biotechnology journal · 2026Article
- Article
- CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA.Nature biotechnology · 2022Article
- Resolving the subtle details of human DNA alkyltransferase lesion search and repair mechanism by single-molecule studies.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Flexible TALEs for an expanded use in gene activation, virulence and scaffold engineering.Nucleic acids research · 2022Article
- Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex.Communications biology · 2021Article
- TALEN outperforms Cas9 in editing heterochromatin target sites.Nature communications · 2021Article
- Tuning up Transcription Factors for Therapy.Molecules (Basel, Switzerland) · 2020Review
- Divalent cations promote TALE DNA-binding specificity.Nucleic acids research · 2020Article
- Additive manufacturing of laminar flow cells for single-molecule experiments.Scientific reports · 2019Article
- Single-molecule visualization reveals the damage search mechanism for the human NER protein XPC-RAD23B.Nucleic acids research · 2019Article
- Facilitated diffusion of Argonaute-mediated target search.RNA biology · 2019Review
- Quantifying the two-state facilitated diffusion model of protein-DNA interactions.Nucleic acids research · 2019Article
- Split-TALE: A TALE-Based Two-Component System for Synthetic Biology Applications in Planta.Plant physiology · 2019Article
- Functional instability allows access to DNA in longer transcription Activator-Like effector (TALE) arrays.eLife · 2019Article
- Polarized displacement by transcription activator-like effectors for regulatory circuits.Nature chemical biology · 2019Article
- Breaking the speed limit with multimode fast scanning of DNA by Endonuclease V.Nature communications · 2018Article
- Mechanisms of Protein Search for Targets on DNA: Theoretical Insights.Molecules (Basel, Switzerland) · 2018Article
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Abstract
Transcription activator-like effector (TALE) proteins are a class of programmable DNA-binding proteins for which the fundamental mechanisms governing the search process are not fully understood. Here we use single-molecule techniques to directly observe TALE search dynamics along DNA templates. We find that TALE proteins are capable of rapid diffusion along DNA using a combination of sliding and hopping behaviour, which suggests that the TALE search process is governed in part by facilitated diffusion. We also observe that TALE proteins exhibit two distinct modes of action during the search process-a search state and a recognition state-facilitated by different subdomains in monomeric TALE proteins. Using TALE truncation mutants, we further demonstrate that the N-terminal region of TALEs is required for the initial non-specific binding and subsequent rapid search along DNA, whereas the central repeat domain is required for transitioning into the site-specific recognition state.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.