ArticleBiomedical microdevices2015
Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.
Article in Biomedical microdevices, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Advanced strategies for single embryo selection in assisted human reproduction: A review of clinical practice and research methods.Clinical and experimental reproductive medicine · 2025Article
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
- Article
- Exploiting Microfluidics for Extracellular Vesicle Isolation and Characterization: Potential Use for Standardized Embryo Quality Assessment.Frontiers in veterinary science · 2020Review
- Workshop report: Cryopreservation of aquatic biomedical models.Cryobiology · 2019Article
- A microfluidic device for motility and osmolality analysis of zebrafish sperm.Biomedical microdevices · 2018Article
- Addressing Reproducibility in Cryopreservation, and Considerations Necessary for Commercialization and Community Development in Support of Genetic Resources of Aquatic Species.Journal of the World Aquaculture Society · 2018Article
- Fish Sperm Assessment Using Software and Cooling Devices.Journal of visualized experiments : JoVE · 2018Article
- Sperm activation through orbital and self-axis revolutions using an artificial cilia embedded serpentine microfluidic platform.Scientific reports · 2018Article
- Designing 3-Dimensional In Vitro Oviduct Culture Systems to Study Mammalian Fertilization and Embryo Production.Annals of biomedical engineering · 2017Review
- Three-dimensional printing with polylactic acid (PLA) thermoplastic offers new opportunities for cryobiology.Cryobiology · 2016Article
- Microfluidics for cryopreservation.Biotechnology advancesReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Sperm cell activation plays a critical role in a range of biological and engineering processes, from fertilization to cryopreservation protocol evaluation. Across a range of species, ionic and osmotic effects have been discovered that lead to activation. Sperm cells of zebrafish (Danio rerio) initiate motility in a hypoosmotic environment. In this study, we employ a microfluidic mixer for the purpose of rapidly diluting the extracellular medium to initiate the onset of cell motility. The use of a microchannel offers a rapid and reproducible mixing profile throughout the device. This greatly reduces variability from trial to trial relative to the current methods of analysis. Coupling these experiments with numerical simulations, we were able to investigate the dynamics of intracellular osmolality as each cell moves along its path through the micromixer. Our results suggest that intracellular osmolality, and hence intracellular ion concentration, only slightly decreases, contrary to the common thought that larger changes in these parameters are required for activation. Utilizing this framework, microfluidics for controlled extracellular environments and associated numerical modeling, has practical applicability in standardizing high-throughput aquatic sperm activation, and more fundamentally, investigations of the intracellular environment leading to motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.