ArticleIranian biomedical journal2015
Effect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells.
Article in Iranian biomedical journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Exploring the Role of SPARC in Atherosclerosis: Mechanisms and Potential Implications.Current atherosclerosis reports · 2025Review
- Dichotomitin promotes osteoblast differentiation and improves osteoporosis by inhibiting oxidative stress.Journal of orthopaedic surgery and research · 2025Article
- Multi-Modality Imaging of Atheromatous Plaques in Peripheral Arterial Disease: Integrating Molecular and Imaging Markers.International journal of molecular sciences · 2023Review
- Upregulation of IL-8, osteonectin, and myonectin mRNAs by intermittent hypoxia via OCT1- and NRF2-mediated mechanisms in skeletal muscle cells.Journal of cellular and molecular medicine · 2022Article
- Probucol promotes osteoblasts differentiation and prevents osteoporosis development through reducing oxidative stress.Molecular medicine (Cambridge, Mass.) · 2022Article
- Effects of semaglutide on vascular structure and proteomics in high-fat diet-induced obese mice.Frontiers in endocrinology · 2022Article
- Inflammation and Vascular Calcification Causing Effects of Oxidized HDL are Attenuated by Adiponectin in Human Vascular Smooth Muscle Cells.International journal of molecular and cellular medicine · 2019Article
- Identification of coordinately regulated microRNA-gene networks that differ in baboons discordant for LDL-cholesterol.PloS one · 2019Article
- Angiopoietin-like 2 has auxo-action in atherosclerosis by promoting atherosclerotic calcification.International journal of clinical and experimental pathology · 2017Article
- A Molecular View of Pathological Microcalcification in Breast Cancer.Journal of mammary gland biology and neoplasia · 2016Review
- Oxidative stress and age-related changes in T cells: is thalassemia a model of accelerated immune system aging?Central-European journal of immunology · 2016Review
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4 authors at 1 institution in 1 country.
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Abstract
backgroundVascular calcification is an important stage in atherosclerosis. During this stage, vascular smooth muscle cells (VSMC) synthesize many osteogenic factors such as osteonectin (encoded by SPARC). Oxidative stress plays a critical role in atherosclerosis progression, and its accumulation in the vascular wall stimulates the development of atherosclerosis and vascular calcification. The osteonectin overexpression has been observed in the arterial wall during the course of atherosclerosis. However, the regulatory mechanism of oxidized low density lipoprotein (oxLDL)-mediated vascular calcification remains to be clarified. The aim of this study was to investigate the effect of oxLDL on the osteonectin gene expression through the Runx2 transcription factor.
methodsIn this experimental study, VSMC were cultured in F-12K media and then treated with oxLDL. The expression of Runx2 and osteonectin genes was determined by real-time PCR method. Protein levels were investigated by the western blotting technique. The Runx2 gene was knocked down using siRNA in order to determine whether Runx2 regulates the osteonectin expression in VSMC induced by oxLDL. Then transfected cells were treated with oxLDL, and the expression levels of Runx2 and osteonectin were determined again.
resultsoxLDL was found to increase Runx2 and osteonectin gene expression (4.8±0.47- and 9.2±1.96-fold, respectively) after 48 h. Western blotting analysis confirmed the induced levels of Runx2 and osteonectin proteins. However, oxLDL-induced osteonectin expression was not observed to be blocked by Runx2 knockdown.
conclusionThe up-regulation of osteonectin by oxLDL is independent of Runx2, and it may be mediated by other transcription factors.
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