Evidence map›Paper›PMID 26025660›Full record

ArticleNeuropharmacology2015

Co-operative binding assay for the characterization of mGlu4 allosteric modulators.

Pekka Poutiainen, Kun-Eek Kil, Zhaoda Zhang, Darshini Kuruppu, Bakhos Tannous, Anna-Liisa Brownell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuropharmacology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. PET imaging studies to investigate functional expression of mGluR2 using [Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023
    Article
  2. Article
  3. Synthesis and Characterization of Fluorine-18-LabeledJournal of medicinal chemistry · 2020
    Article
  4. Differential Pharmacology and Binding of mGluMolecular pharmacology · 2018
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Pekka PoutiainenAthinoula A. Martinos Biomedical Imaging Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Kun-Eek KilAthinoula A. Martinos Biomedical Imaging Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Zhaoda ZhangAthinoula A. Martinos Biomedical Imaging Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Darshini KuruppuDivision of Surgical Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Bakhos TannousExperimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, MA, USA; Program in Neuroscience, Harvard Medical School, Boston, MA, USA.
Anna-Liisa BrownellAthinoula A. Martinos Biomedical Imaging Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. Electronic address: abrownell@partners.org.
Massachusetts General Hospital · USAthinoula A. Martinos Center for Biomedical Imaging · USHarvard University · US

Funding

Positive Allosteric Modulators as PET Imaging Ligans for mGluR4R01NS100164 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI BROWNELL, ANNA-LIISA, ZHANG, ZHAODA · 2016 to 2020
$3.7M
Positive allosteric modulators as PET imaging ligands for mGluR4R01EB012864 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI BROWNELL, ANNA-LIISA · 2011 to 2014
$2.2M
Targeting Glial mGluR5 in SchizophreniaR01MH091684 · NIMH · MASSACHUSETTS GENERAL HOSPITAL · PI BROWNELL, ANNA-LIISA, CICCHETTI, FRANCESCA · 2010 to 2013
$1.2M
500 MHz NMR Spectrometer for Molecular Imaging ApplicationsS10RR023385 · NCRR · MASSACHUSETTS GENERAL HOSPITAL · PI ROSEN, BRUCE R · 2007 to 2007
$500k
NCRR NIH HHS S10 RR023385NIBIB NIH HHS R01 EB012864NIBIB NIH HHS R01EB012864NIMH NIH HHS R01 MH091684NIMH NIH HHS R01MH091684NINDS NIH HHS R01 NS100164
6 · The paper itself

Abstract

The interest in the role of metabotropic glutamate receptor 4 (mGlu4) in CNS related disorders has increased the need for methods to investigate the binding of allosteric drug candidates. Our aim is to present the first fully characterized in vitro binding assay of mGlu4 positive allosteric modulators (PAMs). Results suggest that mGlu4 PAMs have characteristic co-operative binding with orthosteric glutamate, which offers a notable insight to the further development of mGlu4 targeted therapies.

Indexed as

Allosteric RegulationAnimalsBinding, CompetitiveCHO CellsCricetulusDose-Response Relationship, DrugDrug Evaluation, PreclinicalExcitatory Amino Acid AgentsGlutamic AcidReceptors, Metabotropic GlutamateExcitatory Amino Acid AgentsGlutamic Acidmetabotropic glutamate receptor 4Receptors, Metabotropic GlutamateBinding affinityMetabotropic glutamate receptormGlu(4)Parkinson disease

Identifiers

PMID26025660
PMCPMC4537392
OpenAlexW367968289

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.